铜绿假单胞菌噬菌体PP7在3.7埃分辨率下的三维结构。

The three-dimensional structure of bacteriophage PP7 from Pseudomonas aeruginosa at 3.7-A resolution.

作者信息

Tars K, Fridborg K, Bundule M, Liljas L

机构信息

Department of Cell and Molecular Biology, Uppsala University, Uppsala, S-751 24, Sweden.

出版信息

Virology. 2000 Jul 5;272(2):331-7. doi: 10.1006/viro.2000.0373.

DOI:10.1006/viro.2000.0373

PMID:10873776

Abstract

The three-dimensional structure of phage PP7 from Pseudomonas aeruginosa has been determined to 3.7-A resolution. A comparison with distantly related small RNA phages showed that the biggest differences were found in the FG loops, forming the contacts around the fivefold and threefold axes. In contrast to the situation in other phages, the FG loops of phage PP7 are very similar in all three subunits. This supports the hypothesis that no switches are needed for the assembly control in these viruses. Some of the most conserved residues lie within the region involved in RNA binding in the related phages MS2 and seem to have the same function.

摘要

铜绿假单胞菌噬菌体PP7的三维结构已确定至3.7埃分辨率。与远亲小RNA噬菌体的比较表明，最大的差异存在于FG环中，这些环在五重轴和三重轴周围形成接触。与其他噬菌体的情况不同，噬菌体PP7的FG环在所有三个亚基中非常相似。这支持了这些病毒的装配控制不需要开关的假设。一些最保守的残基位于相关噬菌体MS2中参与RNA结合的区域内，并且似乎具有相同的功能。