使用冷冻电镜对单链 RNA 噬菌体和宿主受体进行结构测定的纯化。
Purification of Single-Stranded RNA Bacteriophages and Host Receptors for Structural Determination Using Cryo-Electron Microscopy.
机构信息
Center for Phage Technology, Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, USA.
出版信息
Methods Mol Biol. 2024;2793:185-204. doi: 10.1007/978-1-0716-3798-2_13.
Single-stranded RNA bacteriophages (ssRNA phages) are small viruses with a compact genome (~3-4 kb) that infect gram-negative bacteria via retractile pili. These phages have been applied in various fields since their discovery approximately 60 years ago. To understand their biology, it is crucial to analyze the structure of mature virions. Cryo-electron microscopy (cryo-EM) has been employed to determine the structures of two ssRNA phages, MS2 and Qβ. This chapter presents a method for purifying these two phages and their receptor, the F-pilus, to allow examination using cryo-EM.
单链 RNA 噬菌体（ssRNA 噬菌体）是一种小型病毒，其基因组紧凑（约 3-4kb），通过可收缩的菌毛感染革兰氏阴性菌。自大约 60 年前发现以来，这些噬菌体已在各个领域得到应用。为了了解它们的生物学特性，分析成熟病毒粒子的结构至关重要。冷冻电子显微镜（cryo-EM）已被用于确定两种 ssRNA 噬菌体 MS2 和 Qβ的结构。本章介绍了一种纯化这两种噬菌体及其受体 F 菌毛的方法，以便使用 cryo-EM 进行检查。
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