Yang X P, Chiba S
Department of Pharmacology, Shinshu University School of Medicine, Matsumoto, Japan.
Jpn J Pharmacol. 2000 Jan;82(1):71-3. doi: 10.1254/jjp.82.71.
We tried to induce selective perivascular purinergic nerve stimulation in isolated canine splenic arterial preparations, using the cannula insertion method. Under the conditions of periarterial electrical stimulation (ES), i.e., trains of 1, 3 and 10 pulses, 1-ms pulse duration and 10-V amplitude at 1 Hz, monophasic vasoconstriction was consistently induced. The ES-induced vasoconstriction was not influenced by prazosin in doses that completely inhibited noradrenaline-induced vasoconstrictions, but it was suppressed by alpha,beta-methylene ATP, a P2X purinoceptor desensitizer. Thus, it is indicated that a selective purinergic transmitter release is readily obtained in the isolated splenic arterial preparation.
我们试图采用插管插入法，在离体犬脾动脉制剂中诱导选择性血管周围嘌呤能神经刺激。在动脉周围电刺激（ES）条件下，即1、3和10个脉冲的串刺激，脉冲持续时间1毫秒，幅度10伏，频率1赫兹，持续诱导单相血管收缩。ES诱导的血管收缩不受完全抑制去甲肾上腺素诱导的血管收缩剂量的哌唑嗪影响，但被P2X嘌呤受体脱敏剂α,β-亚甲基ATP抑制。因此，表明在离体脾动脉制剂中很容易获得选择性嘌呤能递质释放。
