O'Connell K M, Rogan M T
Department of Biological Sciences, University of Salford, UK.
Parasitology. 2000 Jun;120 ( Pt 6):649-55. doi: 10.1017/s0031182099005971.
This study examines the effects of Taenia crassiceps cysticerci on the viability of a human T lymphocyte cell line (Jurkat). Both budding and non-budding T. crassiceps metacestodes were cultured over 24 and 48 h in the presence of Jurkat cells. Cell viability decreased with increasing numbers of cysticerci, particularly budding cysticerci. Single cell gel electrophoresis (comet) analysis, which grades DNA damage, showed a significant increase in apoptosis at 24 and 48 h. The morphology of treated cells was determined using acridine orange with the classical morphology of apoptotic bodies seen to increase with increasing cysticerci numbers over time. These results indicate that parasite-induced apoptosis occurs during murine cysticercosis. Such a mechanism may be important in survival of other metacestode infections of medical or veterinary importance.
本研究考察了肥胖带绦虫囊尾蚴对人T淋巴细胞系（Jurkat细胞）活力的影响。将出芽和未出芽的肥胖带绦虫中期绦虫在有Jurkat细胞存在的情况下培养24小时和48小时。细胞活力随着囊尾蚴数量的增加而降低，尤其是出芽的囊尾蚴。用于评估DNA损伤程度的单细胞凝胶电泳（彗星试验）分析显示，在24小时和48小时时凋亡显著增加。使用吖啶橙确定处理后细胞的形态，可见凋亡小体的典型形态随着囊尾蚴数量的增加和时间的推移而增多。这些结果表明，在鼠囊尾蚴病期间会发生寄生虫诱导的凋亡。这种机制可能对于其他具有医学或兽医学重要性的中期绦虫感染的存活至关重要。
