肥胖带绦虫囊尾蚴病：易感和抗性BALB/c小鼠亚系中的免疫反应

Taenia crassiceps cysticercosis: immune response in susceptible and resistant BALB/c mouse substrains.

作者信息

López-Briones Sergio, Lamoyi Edmundo, Fragoso Gladis, Soloski Mark J, Sciutto Edda

机构信息

Department of Immunology, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70-228, 04510 Mexico City, Mexico.

出版信息

Parasitol Res. 2003 Jun;90(3):236-42. doi: 10.1007/s00436-003-0848-z. Epub 2003 Mar 12.

DOI:10.1007/s00436-003-0848-z

PMID:12783314

Abstract

Taenia crassiceps can naturally and experimentally infect rodents in which they reproduce by budding. Differences in the susceptibility to T. crassiceps cysticercosis were found between two BALB/c substrains: BALB/cAnN (susceptible) and BALB/cJ (resistant). In chimeric mice, resistance was transferred to susceptible mice with bone marrow cells from the resistant mice, which argues in favor of an immune mediation of the resistant phenotype. To further explore the immune response that could underlie these differences in susceptibility, the specific cellular immune response elicited by the parasite was explored in both substrains. An increased proliferative response and IL-2 levels were induced by cysticercal antigens only in splenocytes from resistant mice. A decrease in the percentage of CD4(+) (11.1%), CD8(+) (17.5%) was found in splenocytes from susceptible BALB/cAnN mice. A study of the TCRV beta repertoire revealed a significant decrease in V beta 2 in both CD4(+) and CD8(+) splenocytes only in the susceptible BALB/cAnN strain.

摘要

肥胖带绦虫可自然感染并通过实验感染啮齿动物，在这些动物中它通过出芽进行繁殖。在两种BALB/c亚系小鼠（BALB/cAnN，易感；BALB/cJ，抗性）之间发现了对肥胖带绦虫囊尾蚴病易感性的差异。在嵌合小鼠中，抗性通过将抗性小鼠的骨髓细胞转移到易感小鼠而得以传递，这表明抗性表型存在免疫介导作用。为了进一步探究可能导致这些易感性差异的免疫反应，在两个亚系中都探索了寄生虫引发的特异性细胞免疫反应。仅在抗性小鼠的脾细胞中，囊尾蚴抗原诱导了增殖反应增强和白细胞介素-2水平升高。在易感的BALB/cAnN小鼠的脾细胞中，发现CD4(+)（11.1%）、CD8(+)（17.5%）细胞百分比降低。对TCRVβ库的研究显示，仅在易感的BALB/cAnN品系中，CD4(+)和CD8(+)脾细胞中的Vβ2显著减少。