Bortoluzzi S, Di Paolo M L, Rigo A, Vianello F, Zennaro L
Department of Biological Chemistry, University of Padova.
Boll Soc Ital Biol Sper. 1998 Jul-Aug;74(7-8):61-6.
Glucose Oxidase (GOD) has been covalently bound to functionalized glass cover slips. The surface density of immobilized GOD molecules was measured by a method based on the amperometric determination of Flavin Adenine Dinucleotide (FAD). Atomic Force Microscopy (AFM) images, obtained in aqueous solution for the covalently bound enzyme, show a monomolecular layer of the enzyme on a functionalized glass surface. The catalytic constants were measured for the immobilized GOD and compared with those of the free enzyme.
