Styriak I, Pristas P, Javorský P
Institute of Animal Physiology of the Slovak Academy of Sciences, Kosice.
Res Microbiol. 2000 May;151(4):285-9. doi: 10.1016/s0923-2508(00)00148-0.
A strong bias against GATC sites was observed in the genome of phage F4, a lytic Streptococcus bovis bacteriophage. Only three GATC sites were found within the 60.4-kbp genome of this phage. The comparative lack of GATC sequences within the F4 genome was probably not due to dam methylation, as no modification within this site was detected using methylation-sensitive isoschizomer pair restriction endonuclease analysis. The short oligonucleotide composition of available S. bovis DNA sequences suggested the existence of an unknown mechanism for counterselection of GATC sites in S. bovis bacteriophages.
在溶菌性牛链球菌噬菌体F4的基因组中观察到对GATC位点的强烈偏好。在该噬菌体60.4-kbp的基因组中仅发现三个GATC位点。F4基因组中GATC序列相对较少可能并非由于dam甲基化，因为使用甲基化敏感的同裂酶对限制性内切酶分析未检测到该位点的修饰。可用的牛链球菌DNA序列的短寡核苷酸组成表明，牛链球菌噬菌体中存在一种未知的GATC位点反选择机制。
