在猪链球菌2型强毒株中鉴定出一种可诱导的噬菌体。
Identification of an inducible bacteriophage in a virulent strain of Streptococcus suis serotype 2.
作者信息
Harel J, Martinez G, Nassar A, Dezfulian H, Labrie S J, Brousseau R, Moineau S, Gottschalk M
机构信息
Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal C.P. 5000, St-Hyacinthe, Quebec J2S 7C6, Canada .
出版信息
Infect Immun. 2003 Oct;71(10):6104-8. doi: 10.1128/IAI.71.10.6104-6108.2003.
Streptococcus suis infection is considered to be a major problem in the swine industry worldwide. Most virulent Canadian isolates of S. suis serotype 2 do not produce the known virulence markers for this pathogen. PCR-based subtraction hybridization was adapted to isolate unique DNA sequences which were specific to virulent strains of S. suis isolated in Canada. Analysis of some subtracted DNA clones revealed significant homology with bacteriophages of gram-positive bacteria. An inducible phage (named Ss1) was observed in S. suis following the incubation of the virulent strain 89-999 with mitomycin C. Phage Ss1 has a long noncontractile tail and a small isometric nucleocapsid and is a member of the Siphoviridae family. Ss1 phage DNA appears to be present in most Canadian S. suis strains tested in this study, which were isolated from diseased pigs or had proven virulence in mouse or pig models. To our knowledge, this is the first report of the isolation of a phage in S. suis.
猪链球菌感染被认为是全球养猪业的一个主要问题。加拿大大多数2型猪链球菌的强毒株不产生该病原体已知的毒力标记。基于聚合酶链反应（PCR）的消减杂交技术被用于分离加拿大分离的猪链球菌强毒株特有的独特DNA序列。对一些消减DNA克隆的分析显示与革兰氏阳性菌的噬菌体有显著同源性。在用丝裂霉素C培养强毒株89-999后，在猪链球菌中观察到一种可诱导噬菌体（命名为Ss1）。噬菌体Ss1有一条长的非收缩性尾巴和一个小的等轴核衣壳，属于长尾噬菌体科。在本研究中测试的大多数加拿大猪链球菌菌株中似乎都存在Ss1噬菌体DNA，这些菌株是从患病猪中分离出来的，或者在小鼠或猪模型中已证明具有毒力。据我们所知，这是关于在猪链球菌中分离出噬菌体的首次报道。
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