Gamian E, Kochman A, Rabczyński J, Burczak K
Katedra i Zakład Anatomii Patologicznej AM we Wrocławiu.
Polim Med. 1999;29(1-2):3-20.
The application of semipermeable membranes for immunoisolation of living pancreatic islets is an interesting alternative for treatment in diabetes mellitus patients. The aim of this work was in vivo evaluation of biocompatibility of poly(vinyl alcohol) (PVA) macrocapsules and of the polyethylene foil itself as well. To establish the value of the artificial prosthesis made from settled with viable pancreatic islets (PVA) macrocapsules the glucose plasma levels were analyzed versus time after implantation into peritoneal cavity of rats with alloxane-induced diabetes. The results of the experiment revealed the progressive disadvantageous alterations of PVA hydrogel membrane resulting in decrease of permeability of albumin and glucose. The histologic examination of the cellular enzyme activities to estimate the intensity of inflammation in surrounding tissues showed changes typical or chronic proliferative inflammation. The correct function of artificial prosthesis of the pancreas manifested in normalization glucose plasma level has been observed for from 14 to 42 days after implantation.
将半透膜应用于活的胰岛免疫隔离，是糖尿病患者治疗的一种有趣的替代方法。这项工作的目的是对聚乙烯醇（PVA）大胶囊以及聚乙烯箔本身的生物相容性进行体内评估。为了确定由植入了活的胰岛的（PVA）大胶囊制成的人工假体的价值，在将其植入四氧嘧啶诱导糖尿病大鼠的腹腔后，分析了血浆葡萄糖水平随时间的变化。实验结果显示，PVA水凝胶膜逐渐出现不利变化，导致白蛋白和葡萄糖的通透性降低。通过对细胞酶活性进行组织学检查以评估周围组织炎症强度，结果显示出典型的慢性增殖性炎症变化。在植入后14至42天观察到，胰腺人工假体功能正常，表现为血浆葡萄糖水平正常化。
