Anslinger K, Keil W, Weichhold G, Eisenmenger W
Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Germany.
Int J Legal Med. 2000;113(3):189-92. doi: 10.1007/s004140050296.
The seven Y-chromosomal STRs DYS 19, DYS385 I/II, DYS389 I/II, DYS390, DYS391, DYS392 and DYS393 were amplified using two multiplex PCRs. The optimization of the PCR conditions led to reliable and sensitive systems. Co-amplification of the amelogenin locus was possible in both multiplex systems. In a population sample of 151 Bavarian males, a gene diversity of 0.99 was observed. Sensitivity studies revealed a detection limit of 50 pg DNA per 25 microl reaction volume. PCR experiments with combinations of male/male and male/female DNA showed that in male/male mixtures, the minor component could be detected up to a ratio of 1:15, whereas in male/female mixtures the male component could be found in a higher ratio up to 1:60.
使用两种多重聚合酶链反应（PCR）对7个Y染色体短串联重复序列（STR），即DYS 19、DYS385 I/II、DYS389 I/II、DYS390、DYS391、DYS392和DYS393进行扩增。PCR条件的优化产生了可靠且灵敏的系统。在这两种多重系统中均可以对牙釉蛋白基因座进行共扩增。在151名巴伐利亚男性的群体样本中，观察到基因多样性为0.99。灵敏度研究表明，每25微升反应体积的DNA检测限为50皮克。对男性/男性和男性/女性DNA组合进行的PCR实验表明，在男性/男性混合样本中，次要成分在比例高达1:15时仍可被检测到，而在男性/女性混合样本中，男性成分在比例高达1:60时仍可被检测到。
