Carracedo A, Beckmann A, Bengs A, Brinkmann B, Caglia A, Capelli C, Gill P, Gusmão L, Hagelberg C, Hohoff C, Hoste B, Kihlgren A, Kloosterman A, Myhre Dupuy B, Morling N, O'Donnell G, Parson W, Phillips C, Pouwels M, Scheithauer R, Schmitter H, Schneider P M, Schumm J, Skitsa I, Stradmann-Bellinghausen B, Stuart M, Syndercombe Court D, Vide C
Institute of Legal Medicine, Faculty of Medicine 15705 Santiago de Compostela, Galicia, Spain.
Forensic Sci Int. 2001 Jun 1;119(1):28-41. doi: 10.1016/s0379-0738(00)00395-9.
A collaborative exercise was carried out by the European DNA Profiling Group (EDNAP) in the frame work of the STADNAP program, i.e. standardization of DNA profiling in Europe, in order to evaluate the performance of a Y-chromosome STR pentaplex, which includes the loci DYS19, DYS389 I and II, DYS390 and DYS393 and to determine whether uniformity of results could be achieved among different European laboratories. Laboratories were asked to analyze the five Y-STRs using singleplex and multiplex conditions in three bloodstains and one mixed stain (95% female and 5% male). All the laboratories reported the same results even for the mixed stain included in the exercise. This demonstrates the reproducibility and robustness of Y-chromosome STR typing even with multiplex formats and proves the usefulness of Y-STR systems for analyzing mixed stains with a male component.A total of 930 male samples from 10 different populations from Europe were also analysed for all the loci included in the pentaplex. Eight of these ten populations also included haplotype data. As for single gene analysis, haplotype diversity was higher in Germany and Italy and lower in Western European countries and Finland. Pairwise haplotype analysis shows the Finnish departure from the rest of the populations and a relatively homogeneity in the other European populations with F(ST) estimates lower than 0.05.UPGMA analysis shows an association of Western European population (Ireland, UK, Portugal and Galicia) on the one hand and central European populations on the other.
欧洲DNA分析小组（EDNAP）在STADNAP项目（即欧洲DNA分析标准化项目）框架内开展了一项合作活动，以评估包含DYS19、DYS389 I和II、DYS390以及DYS393基因座的Y染色体STR五重试剂盒的性能，并确定不同欧洲实验室之间能否实现结果的一致性。要求各实验室在三种血迹样本和一种混合样本（95%为女性，5%为男性）中使用单重和多重条件分析这五个Y-STR。即使对于此次活动中包含的混合样本，所有实验室报告的结果也相同。这证明了即使采用多重检测形式，Y染色体STR分型仍具有可重复性和稳健性，并证明了Y-STR系统在分析含有男性成分的混合样本方面的实用性。还对来自欧洲10个不同人群的总共930份男性样本进行了该五重试剂盒中所有基因座的分析。这10个人群中有8个还包含单倍型数据。就单基因分析而言，德国和意大利的单倍型多样性较高，而在西欧国家和芬兰则较低。成对单倍型分析显示芬兰与其他人群存在差异，而其他欧洲人群相对同质，F(ST)估计值低于0.05。UPGMA分析显示，一方面是西欧人群（爱尔兰、英国、葡萄牙和加利西亚），另一方面是中欧人群，二者存在关联。
