Hemsley S, Cole N, Canfield P, Willcox M D
Department of Veterinary Anatomy and Pathology, University of Sydney, Sydney, NSW, 2006, Australia.
Res Vet Sci. 2000 Jun;68(3):207-9. doi: 10.1053/rvsc.1999.0358.
Sub-microlitre volumes of normal koala, mouse, dog, rat and cat tears were fractionated using size exclusion-high performance liquid chromatography (SE - HPLC), giving reproducible profiles which were different for each species. Microlitre volumes of tears were also fractionated using sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS - PAGE), resulting in good separation of individual tear proteins with a species specific distribution. Tears from koalas with conjunctivitis and mice with keratitis were similarly examined and showed mostly quantitative changes. These simple, rapid techniques gave reproducible results and, in contrast to conventional separation techniques, used easily obtainable volumes (as little as 0.75 microl) of tears. Their expansion could allow isola tion, identification and quantitation of individual tear components, enabling effective investigation of changes occurring in disease.
使用尺寸排阻高效液相色谱法（SE - HPLC）对正常考拉、小鼠、狗、大鼠和猫的亚微升体积眼泪进行分离，得到了每种物种都不同的可重复图谱。还使用十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）对微升体积的眼泪进行分离，从而实现了对单个泪液蛋白的良好分离，且具有物种特异性分布。对患有结膜炎的考拉和患有角膜炎的小鼠的眼泪进行了类似检查，结果大多显示为定量变化。这些简单、快速的技术给出了可重复的结果，并且与传统分离技术不同，它们使用的眼泪体积很容易获得（低至0.75微升）。它们的扩展可以实现对单个泪液成分的分离、鉴定和定量，从而能够有效研究疾病中发生的变化。
