Veloso Jéssica Fontes, Brandão Guedes Paula Elisa, Lacerda Luciana Carvalho, Santana Juliano Oliveira, Mora-Ocampo Irma Yuliana, Pirovani Carlos Priminho, Oriá Arianne Pontes, Munhoz Alexandre Dias, Alberto Carlos Renata Santiago
Multidisciplinary Campus of Barra, Federal University of Western Bahia, Av. 23 de Agosto, 873, Assunção, Barra, Bahia, Brazil.
Department of Agricultural and Environmental Sciences, Santa Cruz State University, Rodovia Jorge Amado, km 16, Salobrinho, Ilhéus, Bahia, Brazil.
Vet Med Int. 2021 Jul 15;2021:8708023. doi: 10.1155/2021/8708023. eCollection 2021.
The aim of this study was to investigate the proteins found in tear film of healthy domestic cats. Schirmer tear test strips were used to collect tear samples of twelve healthy cats, which were mixed, centrifuged, and placed in a single 1.5 mL microtube that was frozen at -20°C, until analysis by two-dimensional polyacrylamide gel and mass spectrometry associated with high-performance liquid chromatography. The resulting spectra were analyzed and compared with the Swiss-Prot search tool. Forty peptides were detected in the analyzed protein fragments of 90 spots, with 16 proteins identified. Of these, the authors confirmed what has been already found in other studies: lactotransferrin, serum albumin, allergenic lipocalins, and neutrophil gelatinase-associated lipocalin. Others were considered novel in tear film samples of all species: cyclin-dependent protein kinase, serine/arginine repetitive matrix protein, apelin receptor, secretory protein related to C1q/TNF, Wee1, -1,4 glucan phosphorylase, and WD repeat domain 1. The network was divided into 11 clusters, and a biological function was assigned. Most of the proteins have functions in the defense and maintenance of feline ocular surface homeostasis. Serum albumin is a bottleneck protein, with a high betweenness value. This paper is a pioneer in reporting, in-depth, the tear film proteome of domestic cats.
本研究的目的是调查健康家猫泪膜中发现的蛋白质。使用施密特泪液试验条收集12只健康猫的泪液样本，将样本混合、离心，然后置于一个1.5毫升的微量管中，在-20°C下冷冻，直至通过二维聚丙烯酰胺凝胶电泳和与高效液相色谱相关的质谱分析。对所得光谱进行分析，并与瑞士蛋白质数据库搜索工具进行比较。在90个斑点的分析蛋白片段中检测到40种肽段，鉴定出16种蛋白质。其中，作者证实了其他研究中已发现的蛋白质：乳铁蛋白、血清白蛋白、变应原性脂钙蛋白和中性粒细胞明胶酶相关脂钙蛋白。其他蛋白质在所有物种的泪膜样本中被认为是新发现的：细胞周期蛋白依赖性蛋白激酶、丝氨酸/精氨酸重复基质蛋白、apelin受体、与C1q/TNF相关的分泌蛋白、Wee1、-1,4葡聚糖磷酸化酶和WD重复结构域1。该网络被分为11个簇，并赋予了生物学功能。大多数蛋白质在猫眼表稳态的防御和维持中发挥作用。血清白蛋白是一种瓶颈蛋白，具有较高的中介中心性值。本文首次深入报道了家猫的泪膜蛋白质组。
