Piazuelo E, Jimenez P, Lanas A, García A, Esteva F, Sainz R
Service of Gastroenterology, Research Unit, University Hospital, Zaragoza, Spain.
Eur Surg Res. 2000;32(3):191-6. doi: 10.1159/000008762.
Ulceration is a common feature of inflammatory bowel diseases, where subepithelial cell growth is frequently necessary for resolution. In order to further understand the role of colonic fibroblasts in this process, we have used an in vitro model of wound repair to study the response of human colonic fibroblasts to several growth factors expressed in colonic tissues.
Proliferation was determined by [(3)H]thymidine incorporation into DNA in subconfluent fibroblast cultures. In vitro wound repair was determined in confluent fibroblast monolayers after mechanical denudation. The presence of growth factors secreted by fibroblasts was studied in conditioned medium by heparin affinity chromatography and immunodetection with specific antibodies.
Serum and platelet-derived growth factor (PDGF-BB) induced a dramatic increase in both colonic fibroblast proliferation and closure of wounded cell monolayers. Epidermal growth factor (EGF) stimulated both fibroblast activities, but the effect was less potent. However, colonic fibroblasts did not respond to transforming growth factor-beta(1). Conditioned medium stimulated fibroblast proliferation and wound repair activity, which was reverted by the addition of suramin. Furthermore, a PDGF-like factor was isolated from colonic fibroblast-conditioned medium.
EGF and PDGF-BB promote human colonic fibroblast-dependent wound repair activities. Human colonic fibroblasts may exert an autocrine regulation via the production of growth factors.
溃疡是炎症性肠病的常见特征，上皮下细胞生长对于溃疡愈合通常是必需的。为了进一步了解结肠成纤维细胞在此过程中的作用，我们使用了伤口修复的体外模型来研究人结肠成纤维细胞对结肠组织中表达的几种生长因子的反应。
通过将[³H]胸腺嘧啶掺入亚汇合的成纤维细胞培养物中的DNA来测定增殖。在机械剥脱后，在汇合的成纤维细胞单层中测定体外伤口修复。通过肝素亲和色谱法和用特异性抗体进行免疫检测，研究条件培养基中成纤维细胞分泌的生长因子的存在情况。
血清和血小板衍生生长因子（PDGF-BB）可显著增加结肠成纤维细胞的增殖以及受伤细胞单层的闭合。表皮生长因子（EGF）刺激了两种成纤维细胞活性，但效果较弱。然而，结肠成纤维细胞对转化生长因子-β1无反应。条件培养基刺激了成纤维细胞增殖和伤口修复活性，加入苏拉明后这种活性恢复。此外，从结肠成纤维细胞条件培养基中分离出一种PDGF样因子。
EGF和PDGF-BB促进人结肠成纤维细胞依赖性伤口修复活性。人结肠成纤维细胞可能通过产生生长因子发挥自分泌调节作用。
