Ihn H, Kikuchi K, Soma Y, Sato S, Fujimoto M, Tamaki T, Igarashi A, Takehara K
Department of Dermatology, Faculty of Medicine, University of Tokyo, Japan.
Acta Derm Venereol. 1995 Sep;75(5):367-71. doi: 10.2340/0001555575367371.
We investigated the effects of various growth factors (platelet-derived growth factor (PDGF), epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), transforming growth factor-alpha (TGF-alpha), transforming growth factor-beta 1 (TGF-beta 1), tumor necrosis factor-alpha (TNF-alpha), keratinocyte growth factor (KGF)) on fibroblast attachment to plastic plates. It is thought that cell attachment to plastic plates in vitro may represent the step between cell migration and proliferation in vivo during wound healing. Among the growth factors examined, only PDGF and TGF-beta 1 significantly increased fibroblast attachment to both uncoated and collagen-coated plates in a concentration-dependent manner. The addition of anti-PDGF antibody abolished the enhancing effect of PDGF but not that of TGF-beta 1, suggesting that the effect of TGF-beta 1 is not through the autocrine induction of PDGF-related activities secreted by the fibroblasts themselves. These data suggest that PDGF and TGF-beta 1 regulate fibroblast attachment to the suitable environment in the process of dermal wound healing in vivo.
我们研究了多种生长因子（血小板衍生生长因子（PDGF）、表皮生长因子（EGF）、碱性成纤维细胞生长因子（bFGF）、转化生长因子-α（TGF-α）、转化生长因子-β1（TGF-β1）、肿瘤坏死因子-α（TNF-α）、角质形成细胞生长因子（KGF））对成纤维细胞附着于塑料培养皿的影响。体外细胞附着于塑料培养皿被认为可能代表了体内伤口愈合过程中细胞迁移和增殖之间的步骤。在所检测的生长因子中，只有PDGF和TGF-β1能以浓度依赖的方式显著增加成纤维细胞在未包被和胶原包被培养皿上的附着。添加抗PDGF抗体消除了PDGF的增强作用，但未消除TGF-β1的增强作用，这表明TGF-β1的作用不是通过成纤维细胞自身分泌的PDGF相关活性的自分泌诱导实现的。这些数据表明，在体内皮肤伤口愈合过程中，PDGF和TGF-β1调节成纤维细胞附着于适宜的环境。
