McKinnell R G, Picciano D J, Krieg R E
Lab Anim Sci. 1976 Dec;26(6 Pt 1):932-5.
Sperm released into the urine of adult male frogs, Rana pipiens, after intraperitoneal injection of human chorionic gonadotropin, were used to fertilize freshly ovulated eggs. Repeated spermiations from the same animal were induced with repeated injections of the hormone at intervals of four to 11 days. Individual frogs were induced to spermiate up to four times, with fertile sperm ensuing each time. Development of zygotes produced by this method did not differ from the development of ova fertilized by conventional methods.
对成年豹蛙（Rana pipiens）腹腔注射人绒毛膜促性腺激素后，从其尿液中收集精子，用于使刚排出的卵子受精。每隔4至11天重复注射该激素，诱导同一动物多次排精。个别青蛙最多被诱导排精4次，每次都能产生可育精子。通过这种方法产生的受精卵的发育与传统方法受精的卵子的发育没有差异。
