Nishimura H, Niwa K, Miyake M, Iritani A
J Exp Zool. 1982 Sep 20;223(1):75-8. doi: 10.1002/jez.1402230112.
Rat epididymal spermatozoa, at a concentration of 40-65 X 10(4) cells/ml, were preincubated for 5-5, 5 hr and then added to eggs from superovulated, immature rats. By 10-12 hr after insemination, 86% of the eggs had undergone fertilization, but many of them (41%) were polyspermic. When sperm were preincubated at almost the same concentration and for the time stated above, but then diluted to 1-3 X 10(4) cells/ml before insemination, the number of fertilized eggs and the incidence of polyspermy were both reduced, being 56% and 5%, respectively. When a sperm concentration of 1-3 X 10(4) cells/ml was used both during preincubation and at insemination, the fertilization rate of 60% or more, with polyspermy not in excess of 10%, a sperm concentration of 30-100 X 10(4) cells/ml during preincubation and then diluted to approximately 1-3 X 10(4) cells/ml at insemination appears to be optimal.
将浓度为40 - 65×10⁴个细胞/毫升的大鼠附睾精子预孵育5 - 5.5小时，然后添加到超排卵的未成熟大鼠的卵子中。授精后10 - 12小时，86%的卵子受精，但其中许多（41%）是多精受精。当精子以几乎相同的浓度和上述时间进行预孵育，但在授精前稀释至1 - 3×10⁴个细胞/毫升时，受精卵数量和多精受精发生率均降低，分别为56%和5%。当预孵育和授精时均使用1 - 3×10⁴个细胞/毫升的精子浓度时，受精率达60%或更高，多精受精不超过10%，预孵育时精子浓度为30 - 100×10⁴个细胞/毫升，然后在授精时稀释至约1 - 3×10⁴个细胞/毫升似乎是最佳的。
