Noborg U, Gusdal A, Horal P, Lindh M
Department of Clinical Virology, Göteborg University, Gothenburg, Sweden.
Scand J Infect Dis. 2000;32(3):249-52. doi: 10.1080/00365540050165866.
Subjects with serological markers for a past HBV infection may still have HBV DNA in their serum, but the levels of viraemia in such cases are not known. In the present study, of 63 consecutive HBsAg-negative, anti-HBc-positive serum samples with or without anti-HBs, 20 were HBV DNA-positive as analysed by a highly sensitive quantitative PCR, the Cobas Amplicor HBV Monitor test. However, all of these 20 samples had viraemia levels below 1000 copies/ml, compared with median viraemia levels of 10(8.6) and 10(4.3) copies/ml, respectively, in 98 HBeAg-positive and 124 HBeAg-negative HBsAg carriers. There was no difference in viraemia between subjects with anti-HBc alone compared with both anti-HBs and anti-HBc, nor between those with or without hepatitis C virus antibodies. The findings indicate that HBsAg-negative subjects may retain a low infectivity. Their risk for progressive liver damage is probably low, but this deserves further study.
有既往乙肝病毒（HBV）感染血清学标志物的受试者血清中可能仍有HBV DNA，但此类情况下的病毒血症水平尚不清楚。在本研究中，通过高灵敏度定量聚合酶链反应（PCR）即Cobas Amplicor HBV Monitor检测法分析了63份连续的HBsAg阴性、抗-HBc阳性且有或无抗-HBs的血清样本，其中20份为HBV DNA阳性。然而，这20份样本的病毒血症水平均低于1000拷贝/毫升，相比之下，98例HBeAg阳性和124例HBeAg阴性HBsAg携带者的病毒血症中位数水平分别为10(8.6)和10(4.3)拷贝/毫升。单独抗-HBc的受试者与同时有抗-HBs和抗-HBc的受试者之间，以及有无丙型肝炎病毒抗体的受试者之间，病毒血症无差异。研究结果表明，HBsAg阴性受试者可能仍具有低传染性。他们发生进行性肝损伤的风险可能较低，但这值得进一步研究。
