Chamorro S, Revilla C, Alvarez B, López-Fuertes L, Ezquerra A, Domínguez J
Centro de Investigación en Sanidad Animal (INIA), Madrid, Spain.
Immunobiology. 2000 May;202(1):82-93. doi: 10.1016/S0171-2985(00)80055-8.
We have recently described the existence of two subsets of porcine monocytes based on the expression of CD163. In this study we compare the expression of a number of cell surface antigens in CD163+ and CD163- monocyte subsets using three-color flow cytometry. These monocyte subsets show differences with respect to the expression of MHC class II antigens (SLA-DR and DQ) and a variety of adhesion molecules (CD11a, wCD11c, wCD29, CD49d) that are expressed at higher levels on CD163+ monocytes, and of CD14 that is higher expressed by CD 163- cells. These differences on phenotype could reflect differences in the ability of these two subsets to migrate to tissues and may account for the higher allostimulatory capacity of CD163+ cells. In some aspects, the phenotype of CD163+ monocytes resembles that of mature macrophages. In vitro serum-induced maturation of monocytes into macrophages lead to the expression of SWC9 together with an increase in the expression of CD163 and a reduction in that of CD14. These results delineate a maturation pathway where CD14hiCD163-SWC9- monocytes develop into CD14loCD163+SWC9- monocytes and these cells into CD14loCD163+SWC9+ macrophages.
我们最近基于CD163的表达描述了猪单核细胞的两个亚群的存在。在本研究中，我们使用三色流式细胞术比较了CD163 +和CD163-单核细胞亚群中多种细胞表面抗原的表达。这些单核细胞亚群在MHC II类抗原（SLA-DR和DQ）以及多种粘附分子（CD11a、wCD11c、wCD29、CD49d）的表达方面存在差异，这些分子在CD163 +单核细胞上表达水平更高，而CD14在CD163-细胞中表达更高。表型上的这些差异可能反映了这两个亚群迁移到组织的能力差异，并且可能解释了CD163 +细胞更高的同种异体刺激能力。在某些方面，CD163 +单核细胞的表型类似于成熟巨噬细胞。体外血清诱导单核细胞成熟为巨噬细胞会导致SWC9的表达以及CD163表达的增加和CD14表达的减少。这些结果描绘了一条成熟途径，其中CD14hiCD163-SWC9-单核细胞发育为CD14loCD163 + SWC9-单核细胞，并且这些细胞发育为CD14loCD163 + SWC9 +巨噬细胞。
