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循环白细胞的组成随年龄和黑素瘤发病而变化，在 MeLiM 猪生物医学模型中。

The Composition of Circulating Leukocytes Varies With Age and Melanoma Onset in the MeLiM Pig Biomedical Model.

机构信息

INSERM, U1016, Institut Cochin, Paris, France.

Université Paris-Saclay, INRAE, AgroParisTech, GABI, Jouy-en-Josas, France.

出版信息

Front Immunol. 2020 Feb 28;11:291. doi: 10.3389/fimmu.2020.00291. eCollection 2020.

DOI:10.3389/fimmu.2020.00291
PMID:32180771
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7059855/
Abstract

Immunological research in pigs benefits from many improvements with a direct impact on the veterinary control of pig husbandry and on biomedical models. We compiled the available knowledge to develop gating strategies to monitor simultaneously all blood immune cell types by multicolor flow cytometry in Melanoblastoma-bearing Libechov Minipigs (MeLiM). The MeLiM pig spontaneously develops cutaneous melanomas that regress few months later. We monitored lymphoid and myeloid cell subsets in 3 to 21 weeks old pigs. Interestingly, neutrophils, type III monocytes (CD163 CD14 MHC II) and CD4 CD8α T cells are less abundant in oldest animals in contrast to eosinophils, type II monocytes (CD163 CD14 MHC II), B cells, γδ T cells, CD4 CD8α and CD4 CD8α T cells. Melanoma occurrence led to changes in the blood cell composition. Higher proportions of NK cells, CD4 and CD4 CD8α T cells, and CD21 B cells among B cells are found in young melanoma-bearing piglets, consistent with the immune-mediated spontaneous regression in the MeLiM model.

摘要

猪的免疫学研究受益于许多改进，这些改进直接影响到猪养殖业的兽医控制和生物医学模型。我们汇集了现有知识，制定了门控策略，以便通过多色流式细胞术同时监测携带黑色素瘤的 Libechov 迷你猪（MeLiM）中的所有血液免疫细胞类型。MeLiM 猪会自发地患上皮肤黑色素瘤，几个月后会自行消退。我们监测了 3 至 21 周龄猪的淋巴样和髓样细胞亚群。有趣的是，与嗜酸性粒细胞、II 型单核细胞（CD163+ CD14+ MHC II）、B 细胞、γδ T 细胞、CD4+ CD8α 和 CD4+ CD8α T 细胞相比，年老动物体内的中性粒细胞、III 型单核细胞（CD163+ CD14+ MHC II）和 CD4+ CD8α T 细胞较少。黑色素瘤的发生导致了血细胞组成的变化。在年轻的黑色素瘤携带猪仔中，NK 细胞、CD4 和 CD4+ CD8α T 细胞以及 B 细胞中的 CD21 B 细胞的比例较高，这与 MeLiM 模型中的免疫介导的自发消退一致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0252/7059855/f97f97e9d516/fimmu-11-00291-g0007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0252/7059855/52b863bb2f82/fimmu-11-00291-g0005.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0252/7059855/57efa957a5e5/fimmu-11-00291-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0252/7059855/db447a96a064/fimmu-11-00291-g0003.jpg
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