[Growth and metabolism of hybridoma cells in semicontinuous perfusion cultures].

The growth and metabolism of WuT3 hybridoma cells were studied in semicontinuous perfusion cultures. The growth of cells, utilization of glucose, production of lactate, ammonia and monoclonal antibody were investigated. As the perfusion rate increased from 1.0/d to 2.0/d, the lactate yield coefficient from glucose Ylac/glu decreased 18%, the ammonia yield coefficient from glutamine Yamm/gln decreased 40%, the alanine yield coefficient from glutamine Yala/gln increased 58%, the glycine yield coefficient remained almost constant. This indicate that cells would regulated their metabolic flux at higher cell density, and glutamate produced in quantity and pyruvate would turn into alanine rather than glycine by transamination. As a result, potentially toxic effects were reduced because less ammonia and lactate were produced.