Legrand C, Capiaumont J, Belleville F, Nabet P
Laboratoire de Biochimie Médicale, Faculté de Médecine et IBN, Nancy, France.
Biotechnol Prog. 1993 Nov-Dec;9(6):573-9. doi: 10.1021/bp00024a002.
The effects of industrial whey on cell physiology were studied in a repeated fed-harvest mode using free-suspended murine hybridoma cells. After several days of culture in medium containing 9% whey and 1% fetal calf serum (FCS), cell growth and viability, carbohydrate, amino acid, energy metabolism, and antibody production rates were investigated. Differences were found between cells cultured in whey medium and those cultured in conventional FCS medium. The cell growth obtained in medium supplemented with fresh whey was similar to that obtained in FCS medium. The viability showed an increase for hybridoma cells cultured in whey medium. Glucose consumption rates were similar, whereas the lactate production rate was higher in whey medium. The metabolic uptake rates of glutamine and ammonia increased in whey medium. More alanine, glutamate, glycine, and proline were produced; their production partly came from glutamine and lactate. The consumption rates of branched amino acids changed little; their utilization was higher in whey medium. Finally, antibody productivity was increased about 20% for cells cultured in medium containing whey.
采用自由悬浮的小鼠杂交瘤细胞，以重复补料-收获模式研究了工业乳清对细胞生理学的影响。在含有9%乳清和1%胎牛血清（FCS）的培养基中培养几天后，研究了细胞生长、活力、碳水化合物、氨基酸、能量代谢和抗体产生率。发现乳清培养基中培养的细胞与传统FCS培养基中培养的细胞存在差异。添加新鲜乳清的培养基中获得的细胞生长与FCS培养基中相似。在乳清培养基中培养的杂交瘤细胞活力有所增加。葡萄糖消耗率相似，而乳清培养基中的乳酸产生率更高。乳清培养基中谷氨酰胺和氨的代谢摄取率增加。产生了更多的丙氨酸、谷氨酸、甘氨酸和脯氨酸；它们的产生部分来自谷氨酰胺和乳酸。支链氨基酸的消耗率变化不大；它们在乳清培养基中的利用率更高。最后，在含有乳清的培养基中培养的细胞抗体生产率提高了约20%。
