[几种营养因子对烟草悬浮培养细胞中辅酶Q10含量的影响]

[Effects of a few nutritional factors on content of coenzyme Q10 by tobacco plant cells in suspension culture].

作者信息

Gao X Y, Ye G H, Mu H, Xu F C

机构信息

College of Biotechnology, South China Agr. Univ., Guangzhou.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2000 Jan;16(1):78-81.

PMID:10883282

Abstract

With suspension cultured cells of tobacco (Nicoliana Tobacum L.), effects of inorganic phosphate, nitrogen sources and carbon source on cell growth and CoQ10 content were investigated. It showed that, When treated with 30 g/L of sucrose, the total amount of CoQ10 was highest (8212 g/L); and the cell biomass and CoQ10 content were 19.8 micrograms/L, 414.7 micrograms/g(dwt) respectively. When treated 340 mg/L of KH2PO4, the cell biomass, the content and total amount of CoQ10 were 21.04 g/L, 514.5 micrograms/g(dwt) and 10824 micrograms/L respectively. When treated with the ratio of NH4+/NO3- was 1:2, the cell biomass, the content and total amount of CoQ10 were 21.04 g/L, 514.3 micrograms/g(dwt) and 10,820 micrograms/L respectively. The high ration of NH4+/NO3- was suitable for the formation of CoQ10, but not for the cell growth.

摘要

以烟草（Nicotiana Tobacum L.）悬浮培养细胞为材料，研究了无机磷、氮源和碳源对细胞生长及辅酶Q10含量的影响。结果表明，当蔗糖浓度为30 g/L时，辅酶Q10总量最高（8212 μg/L）；细胞生物量和辅酶Q10含量分别为19.8 μg/L、414.7 μg/g（干重）。当KH2PO4浓度为340 mg/L时，细胞生物量、辅酶Q10含量和总量分别为21.04 g/L、514.5 μg/g（干重）和10824 μg/L。当NH4+/NO3-比例为1:2时，细胞生物量、辅酶Q10含量和总量分别为21.04 g/L、514.3 μg/g（干重）和10820 μg/L。较高的NH4+/NO3-比例适合辅酶Q10的合成，但不利于细胞生长。