Matsushima H, Mukai K, Obara Y, Cai H, Ito Y, Ji C
Department of Ophthalmology, Dokkyo University School of Medicine, Tochigi, Japan.
Nippon Ganka Gakkai Zasshi. 2000 Jun;104(6):377-83.
The cataract in Sprague-Dawley rats injected with selenite is a dense nuclear opacity that appears by 4 or 5 days after selenite injection and becomes irreversible by 7 days. Injection of Wistar rats with selenite resulted in a similar nuclear opacity by 4 or 5 days that began to recover transparency by 7 days. In this report, the cytoplasmic proteins were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in lenses from Sprague-Dawley and Wistar rats at 4 and 7 days after injection.
In the opaque lens cells, degradation of the 31 kDa protein and cytoskeletal proteins (vimentin, spectrin, and actin) was observed during cataract development using SDS-PAGE and western blot analysis. During recovery from opacity, the decreased 31 kDa protein and the vimentin increased.
The results suggest that the 31 kDa protein and the vimentin may be important for recovery of transparency in a reversible model of cataract formation.
给Sprague-Dawley大鼠注射亚硒酸盐后形成的白内障是一种致密的核混浊，在注射亚硒酸盐后4或5天出现，7天后变得不可逆。给Wistar大鼠注射亚硒酸盐后4或5天也出现类似的核混浊，到7天时开始恢复透明。在本报告中，使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对注射后4天和7天的Sprague-Dawley大鼠和Wistar大鼠晶状体中的细胞质蛋白进行了分析。
在不透明晶状体细胞中，使用SDS-PAGE和蛋白质印迹分析发现在白内障形成过程中31 kDa蛋白和细胞骨架蛋白（波形蛋白、血影蛋白和肌动蛋白）发生降解。在从不透明状态恢复过程中，减少的31 kDa蛋白和波形蛋白增加。
结果表明，在白内障形成的可逆模型中，31 kDa蛋白和波形蛋白可能对恢复透明性很重要。
