Matsushima H, Mukai K, Obara Y, Cai H, Ito Y, Ji C
Department of Ophthalmology, Dokkyo University School of Medicine, Dokkyo, Japan
Jpn J Ophthalmol. 2000 Nov 1;44(6):693-694. doi: 10.1016/s0021-5155(00)00256-2.
Background and Purpose: The cataract in Sprague-Dawley rats injected with selenite is a dense nuclear opacity that appears by 4 or 5 days after selenite injection and becomes irreversible by 7 days. Injection of Wistar rats with selenite resulted in a similar nuclear opacity by 4 or 5 days that began to recover transparency by 7 days. In this report, the cytoplasmic proteins were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in lenses from Sprague-Dawley and Wistar rats at 4 and 7 days after injection.Results: In the opaque lens cells, degradation of the 31 kDa protein and cytoskeletal proteins (vimentin, spectrin, and actin) was observed during cataract development using SDS-PAGE and Western blot analysis. During recovery from opacity, the decreased 31 kDa protein and the vimentin increased.Conclusion: The results suggest that the 31 kDa protein and the vimentin may be important for recovery of transparency in a reversible model of cataract formation.
给斯普拉格-道利大鼠注射亚硒酸盐后形成的白内障是一种致密的核混浊，在注射亚硒酸盐后4或5天出现，7天后变得不可逆。给Wistar大鼠注射亚硒酸盐后，4或5天也出现了类似的核混浊，到7天时开始恢复透明。在本报告中，使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对注射后4天和7天的斯普拉格-道利大鼠和Wistar大鼠晶状体中的细胞质蛋白进行了分析。
在不透明晶状体细胞中，使用SDS-PAGE和蛋白质印迹分析观察到在白内障形成过程中31 kDa蛋白和细胞骨架蛋白（波形蛋白、血影蛋白和肌动蛋白）的降解。在从不透明状态恢复过程中，减少的31 kDa蛋白和波形蛋白增加。
结果表明，在白内障形成的可逆模型中，31 kDa蛋白和波形蛋白可能对恢复透明很重要。
