革兰氏阳性菌细胞壁抗原对淋巴细胞刺激及细胞介导细胞毒性作用的研究。

Investigation into the effects of cell wall antigens of gram-positive bacteria on lymphocyte stimulation and on cell-mediated cytotoxicity.

作者信息

Buschmann H, Henrich D

出版信息

Zentralbl Bakteriol Orig A. 1979 Mar;243(1):125-35.

Abstract

The effect of Staph, epidermidis and Bac. subtilis cell walls as well as of cell wall teichoic acid, N-acetyl-muramic acid, N-acetyl-D-glucosamine, D-alanine, and DL-alpha, epsilon-diaminopimelic acid on lymphocyte stimulation and on cell-mediated cytotoxicity has been studied. Bac. subtilis cell wall preparations, N-acetyl-D-glucosamine and teichoic acid showed a slight but significant effect on the mitogenic response of pig lymphocyte cultures. When studied in combination with the mitogens PHA, ConA, and PWM significant synergistic effects were observed with N-acetyl-muramic acid. The most significant stimulation of the in vitro 51Cr-release from labelled P815 tumor cells in the presence of non-sensitized as well as specifically sensitized lymphocytes was exerted by D-alanine.

摘要

已研究了表皮葡萄球菌和枯草芽孢杆菌细胞壁以及细胞壁磷壁酸、N - 乙酰胞壁酸、N - 乙酰 - D - 葡萄糖胺、D - 丙氨酸和DL-α,ε-二氨基庚二酸对淋巴细胞刺激和细胞介导的细胞毒性的影响。枯草芽孢杆菌细胞壁制剂、N - 乙酰 - D - 葡萄糖胺和磷壁酸对猪淋巴细胞培养物的促有丝分裂反应显示出轻微但显著的影响。当与促有丝分裂原PHA、ConA和PWM联合研究时，观察到N - 乙酰胞壁酸有显著的协同作用。在未致敏以及特异性致敏的淋巴细胞存在的情况下，D - 丙氨酸对标记的P815肿瘤细胞的体外51Cr释放具有最显著的刺激作用。