人类淋巴细胞对细菌成分的多克隆抗体形成。
Polyclonal antibody formation of human lymphocytes to bacterial components.
作者信息
Räsänen L, Lehto M, Jokinen I, Leinikki P
出版信息
Immunology. 1986 Aug;58(4):577-81.
The capacity of various bacterial components to induce antibody formation in human lymphocyte cultures was studied in the present investigation. Antibody levels were determined by an enzyme-linked immunosorbent assay (ELISA). Lipopolysaccharide (LPS), bacterial cell walls (CW, isolated from Bacillus subtilis and Staphylococcus aureus Wood 46) and peptidoglycans (PG) appeared to stimulate IgM, IgG and IgA secretion, whereas lysozyme-solubilized PG and teichoic acids (TA) were ineffective. Also, umbilical cord blood lymphocytes produced IgM after stimulation with LPS, CW and PG. Coculture experiments with purified lymphocytes and monocytes indicated that B-cell differentiation was dependent on both T cells and monocytes, and that T-cell derived factors could derived factors could partially substitute for T cells.
本研究探讨了多种细菌成分在人淋巴细胞培养物中诱导抗体形成的能力。通过酶联免疫吸附测定法（ELISA）测定抗体水平。脂多糖（LPS）、细菌细胞壁（CW，从枯草芽孢杆菌和金黄色葡萄球菌Wood 46中分离）和肽聚糖（PG）似乎能刺激IgM、IgG和IgA分泌，而溶菌酶溶解的PG和磷壁酸（TA）则无效。此外，脐带血淋巴细胞在用LPS、CW和PG刺激后产生IgM。纯化淋巴细胞和单核细胞的共培养实验表明，B细胞分化依赖于T细胞和单核细胞，并且T细胞衍生因子可以部分替代T细胞。
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