关于DNA病毒是真核生物复制蛋白起源的一种假说。
A hypothesis for DNA viruses as the origin of eukaryotic replication proteins.
作者信息
Villarreal L P, DeFilippis V R
机构信息
Departments of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA.
出版信息
J Virol. 2000 Aug;74(15):7079-84. doi: 10.1128/jvi.74.15.7079-7084.2000.
The eukaryotic replicative DNA polymerases are similar to those of large DNA viruses of eukaryotic and bacterial T4 phages but not to those of eubacteria. We develop and examine the hypothesis that DNA virus replication proteins gave rise to those of eukaryotes during evolution. We chose the DNA polymerase from phycodnavirus (which infects microalgae) as the basis of this analysis, as it represents a virus of a primitive eukaryote. We show that it has significant similarity with replicative DNA polymerases of eukaryotes and certain of their large DNA viruses. Sequence alignment confirms this similarity and establishes the presence of highly conserved domains in the polymerase amino terminus. Subsequent reconstruction of a phylogenetic tree indicates that these algal viral DNA polymerases are near the root of the clade containing all eukaryotic DNA polymerase delta members but that this clade does not contain the polymerases of other DNA viruses. We consider arguments for the polarity of this relationship and present the hypothesis that the replication genes of DNA viruses gave rise to those of eukaryotes and not the reverse direction.
真核生物的复制性DNA聚合酶与真核生物的大型DNA病毒以及细菌T4噬菌体的DNA聚合酶相似，但与真细菌的DNA聚合酶不同。我们提出并检验了一个假说，即在进化过程中，DNA病毒的复制蛋白产生了真核生物的复制蛋白。我们选择了来自藻DNA病毒（感染微藻）的DNA聚合酶作为该分析的基础，因为它代表了一种原始真核生物的病毒。我们发现它与真核生物及其某些大型DNA病毒的复制性DNA聚合酶具有显著的相似性。序列比对证实了这种相似性，并确定了聚合酶氨基末端存在高度保守的结构域。随后构建的系统发育树表明，这些藻类病毒DNA聚合酶位于包含所有真核生物DNA聚合酶δ成员的进化枝的根部附近，但该进化枝不包含其他DNA病毒的聚合酶。我们考虑了这种关系极性的论据，并提出假说，即DNA病毒的复制基因产生了真核生物的复制基因，而不是相反的方向。
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