Chen F, Suttle C A
Department of Marine Science, University of Texas at Austin, Port Aransas 78373, USA.
Virology. 1996 May 1;219(1):170-8. doi: 10.1006/viro.1996.0234.
In order to examine genetic relatedness among viruses that infect microalgae, DNA polymerase gene (DNA pol) fragments were amplified and sequenced from 13 virus clones that infect three genera of distantly related microalgae (Chlorella strains NC64A and Pbi, Micromonas pusilla and Chrysochromulina spp.). Phylogenetic trees based on DNA pol sequences and hybridization of total genomic DNA showed similar branching patterns. Genetic relatedness calculated from the hybridization and sequence data showed good concordance (r=0.90), indicating that DNA pol sequences can be used to determine genetic relatedness and infer phylogenetic relationships among these viruses. The phylogenetic tree inferred from the deduced amino acid sequences of DNA pol from 24 dsDNA viruses, including phycodnaviruses, herpesviruses, poxviruses, baculoviruses, and African swine fever virus corresponded well with groupings based on the International Committee on Taxonomy of Viruses. Microalgal viruses are more closely related to each other than to the other dsDNA viruses and form a distinct phyletic group, suggesting that they share a common ancestor and belong to the Phycodnaviridae. Moreover, the Phycodnaviridae are more closely related to the Herpesviridae than to other virus families for which DNA pol sequences are available.
为了研究感染微藻的病毒之间的遗传相关性，从13个感染远缘相关的三个微藻属（小球藻菌株NC64A和Pbi、微小原甲藻和金色藻属）的病毒克隆中扩增并测序了DNA聚合酶基因（DNA pol）片段。基于DNA pol序列构建的系统发育树以及全基因组DNA的杂交显示出相似的分支模式。从杂交和序列数据计算得出的遗传相关性显示出良好的一致性（r = 0.90），表明DNA pol序列可用于确定这些病毒之间的遗传相关性并推断系统发育关系。从包括藻DNA病毒、疱疹病毒、痘病毒、杆状病毒和非洲猪瘟病毒在内的24种双链DNA病毒的DNA pol推导氨基酸序列推断出的系统发育树与基于国际病毒分类委员会的分类结果吻合良好。微藻病毒彼此之间的亲缘关系比与其他双链DNA病毒更密切，并形成一个独特的进化类群，这表明它们有共同的祖先，属于藻DNA病毒科。此外，藻DNA病毒科比其他有DNA pol序列的病毒科与疱疹病毒科的亲缘关系更密切。
