Malik Z, Agam G, Djaldetti M
Acta Haematol. 1979;61(3):138-43. doi: 10.1159/000207646.
The hemin effect on protein synthesis of human granulocytes, lymphocytes and platelets was examined. Hemin added to culture media without serum caused a dose-dependent inhibition of protein synthesis in all three cell types. A cell-specific enhancement of protein-synthesizing capability was observed in 24-hour cultures in the presence of hemin and serum. A marked increase of protein synthesis was found in granulocytes, unchanged in lymphocytes and decreased in platelets. Lymphocytes from patients with chronic lymphatic leukemia (CLL) were moderately inhibited by hemin when incubated in media containing serum, the effect being more pronounced in the presence of freshly disolved doses of hemin. Addition of protoporphyrin IX to cells cultures resulted in a marked suppression of protein synthesis by the three cell types, in all experiments. These results confirm the importance of serum proteins in preventing the inhibitory effects of free hemin and protoporphyrin IX on blood cell protein synthesis. On the other hand, they show a cell-specific enhancement of the protein-synthesizing capacity mediated by hemin.
研究了血红素对人粒细胞、淋巴细胞和血小板蛋白质合成的影响。在无血清的培养基中添加血红素会导致这三种细胞类型的蛋白质合成呈剂量依赖性抑制。在存在血红素和血清的情况下，在24小时培养物中观察到细胞特异性的蛋白质合成能力增强。粒细胞中蛋白质合成显著增加，淋巴细胞中不变，血小板中减少。慢性淋巴细胞白血病（CLL）患者的淋巴细胞在含有血清的培养基中孵育时受到血红素的中度抑制，在存在新溶解剂量的血红素时这种作用更明显。在所有实验中，向细胞培养物中添加原卟啉IX会导致这三种细胞类型的蛋白质合成显著抑制。这些结果证实了血清蛋白在防止游离血红素和原卟啉IX对血细胞蛋白质合成的抑制作用方面的重要性。另一方面，它们显示了由血红素介导的细胞特异性蛋白质合成能力增强。
