Verger C, Sassa S, Kappas A
J Cell Physiol. 1983 Aug;116(2):135-41. doi: 10.1002/jcp.1041160203.
The effects of hemin (Fe-protoporphyrin) and Co-protoporphyrin on cellular growth have been investigated principally in cultured fibroblasts, but also in myoblasts and hepatocytes from chick embryos. In the presence of horse serum in the culture medium, which by itself did not stimulate cell growth appreciably, Co-protoporphyrin stimulated cell attachment while hemin stimulated cell proliferation of fibroblasts. When Co-protoporphyrin and hemin were added together, the most potent stimulation of cell growth, consisting of increases in cell attachment and rapid cell proliferation, was observed. These findings indicate that the two metalloporphyrins have differential and complementary effects on cellular growth in culture, with synthetic Co-protoporphyrin principally affecting cellular attachment and Fe-protoporphyrin stimulating cellular proliferation.
人们主要在培养的成纤维细胞中研究了血红素（铁原卟啉）和钴原卟啉对细胞生长的影响，不过也在鸡胚的成肌细胞和肝细胞中进行了研究。在培养基中存在马血清的情况下（马血清本身并不会显著刺激细胞生长），钴原卟啉促进细胞附着，而血红素促进成纤维细胞的增殖。当同时添加钴原卟啉和血红素时，观察到了对细胞生长最有力的刺激作用，包括细胞附着增加和细胞快速增殖。这些发现表明，这两种金属卟啉对培养中的细胞生长具有不同的互补作用，合成的钴原卟啉主要影响细胞附着，而铁原卟啉刺激细胞增殖。
