基因调控：乳糖操纵子控制区

Genetic regulation: the Lac control region.

作者信息

Dickson R C, Abelson J, Barnes W M, Reznikoff W S

出版信息

Science. 1975 Jan 10;187(4171):27-35. doi: 10.1126/science.1088926.

Abstract

The nucleotide sequence of the lac promoter-operator region has been determined. The 122 base pairs comprising this region include the recognition sites for RNA polymerase, the positive regulatory protein, CAP, and the negative regulatory protein, the repressor. Identification of mutant variants of the sequence combined with the in vitro biochemical studies of others has allowed us to tentatively identify the recognition site for each of these proteins, and to suggest how CAP might act at a distance to affect the interaction of RNA polymerase with the promoter.

摘要

乳糖启动子 - 操纵基因区域的核苷酸序列已被确定。构成该区域的122个碱基对包括RNA聚合酶、正调控蛋白CAP以及负调控蛋白阻遏物的识别位点。对该序列突变变体的鉴定，结合其他人的体外生化研究，使我们能够初步确定这些蛋白质各自的识别位点，并提出CAP可能如何在远距离发挥作用以影响RNA聚合酶与启动子的相互作用。