一种具有用于乳糖操纵子的新型高效启动子的大肠杆菌突变体。
A mutant of Escherichia coli with a new, highly efficient promoter for the lactose operon.
作者信息
Bruenn J, Hollingsworth H
出版信息
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3693-7. doi: 10.1073/pnas.70.12.3693.
Abstract
We have isolated a mutant of E. coli whose maximal rate of synthesis of lac mRNA is 25-fold greater than that of its parental wild-type strain. The mutant also shows alterations in the kinetics of beta-galactosidase synthesis, the functional half-life of beta-galactosidase mRNA, and the properties of lac repressor.
摘要
我们分离出了一株大肠杆菌突变体，其乳糖信使核糖核酸（lac mRNA）的最大合成速率比其亲本野生型菌株高25倍。该突变体在β-半乳糖苷酶合成动力学、β-半乳糖苷酶信使核糖核酸的功能半衰期以及乳糖阻遏物的特性方面也表现出改变。
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本文引用的文献
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KINETICS OF INDUCED ENZYME SYNTHESIS. DETERMINATION OF THE MEAN LIFE OF GALACTOSIDASE-SPECIFIC MESSENGER RNA.诱导酶合成动力学。半乳糖苷酶特异性信使核糖核酸平均寿命的测定。
Biochim Biophys Acta. 1963 Oct 15;76:293-309.
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[Incorporation of structural analogues of amino acids into bacterial proteins during their synthesis in vivo].[在体内细菌蛋白质合成过程中氨基酸结构类似物掺入细菌蛋白质的情况]
Biochim Biophys Acta. 1959 Feb;31(2):378-91. doi: 10.1016/0006-3002(59)90011-3.
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A membrane-filter technique for the detection of complementary DNA.一种用于检测互补DNA的膜过滤技术。
Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6. doi: 10.1016/0006-291x(66)90447-5.
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The ribonuclease activity of crystallized pancreatic deoxyribonuclease.结晶胰脱氧核糖核酸酶的核糖核酸酶活性
Anal Biochem. 1966 Feb;14(2):269-77. doi: 10.1016/0003-2697(66)90137-0.
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Evidence for two sites for initiation of gene expression in the tryptophan operon of Salmonella typhimurium.鼠伤寒沙门氏菌色氨酸操纵子中基因表达起始存在两个位点的证据。
J Mol Biol. 1967 Jun 28;26(3):423-36. doi: 10.1016/0022-2836(67)90313-0.
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The nature of lactose operator constitive mutations.乳糖操纵基因组成型突变的本质。
J Mol Biol. 1971 Jul 28;59(2):273-305. doi: 10.1016/0022-2836(71)90051-9.
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Inactivation and degradation of messenger ribnucleic acid from the lactose operon of Escherichia coli.来自大肠杆菌乳糖操纵子的信使核糖核酸的失活与降解
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