一种具有用于乳糖操纵子的新型高效启动子的大肠杆菌突变体。
A mutant of Escherichia coli with a new, highly efficient promoter for the lactose operon.
作者信息
Bruenn J, Hollingsworth H
出版信息
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3693-7. doi: 10.1073/pnas.70.12.3693.
Abstract
We have isolated a mutant of E. coli whose maximal rate of synthesis of lac mRNA is 25-fold greater than that of its parental wild-type strain. The mutant also shows alterations in the kinetics of beta-galactosidase synthesis, the functional half-life of beta-galactosidase mRNA, and the properties of lac repressor.
摘要
我们分离出了一株大肠杆菌突变体，其乳糖信使核糖核酸（lac mRNA）的最大合成速率比其亲本野生型菌株高25倍。该突变体在β-半乳糖苷酶合成动力学、β-半乳糖苷酶信使核糖核酸的功能半衰期以及乳糖阻遏物的特性方面也表现出改变。
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