Houdijk J G, Kyriazakis I, Jackson F, Huntley J F, Coop R L
Animal Nutrition and Health Department, Animal Biology Division, Scottish Agricultural College, Kings Buildings, EH9 3JG, Edinburgh, UK.
Vet Parasitol. 2000 Jul 24;91(1-2):43-62. doi: 10.1016/s0304-4017(00)00255-7.
Within the overall hypothesis that the periparturient relaxation of immunity (PPRI) to gastrointestinal parasites has a nutritional basis, we studied whether an increased intake of metabolizable protein (MP) could affect the expression of immunity to Teladorsagia (Ostertagia) circumcincta of twin-bearing and -rearing Greyface ewes. Two sets of iso-energetic diets were formulated to provide either 85% (L-) or 130% (H-) of the MP requirements during the last 6 weeks of pregnancy (-P) and the first 6 weeks of lactation (-L). Thirty ewes were allocated to LP or HP. Six ewes of each group were slaughtered after lambing (S(0)); the remaining 24 ewes of each group were allocated to either LL or HL, thus creating four treatment combinations during lactation (LP/LL, LP/HL, HP/LL and HP/HL; n=12). Six ewes of each treatment combination were slaughtered at Week 3 (S(3)) or at Week 6 (S(6)) in lactation. The HP ewes were heavier at lambing, produced heavier lambs, had lower faecal egg counts (FECs), a lower concentration of plasma pepsinogen, a higher concentration of plasma albumin during late pregnancy and more abomasal globule leukocytes (GLs) but similar worm burdens at S(0) compared to the LP ewes. The LP/HL and HP/HL ewes had a higher calculated milk production than the LP/LL and HP/LL ewes. FECs increased for each treatment combination during the first 3 weeks of lactation. This increase concurred with fewer abomasal GLs at S(3) compared to S(0). The LP/HL and HP/HL ewes had a higher proportion of immature worms and more abomasal GLs than the LP/LL and HP/LL ewes at S(6). The FECs continued to increase during the second 3 weeks of lactation for the LP/LL ewes only, which concurred with an elevated plasma pepsinogen concentration and an absence of abomasal GLs at S(6). The plasma pepsinogen concentration decreased rapidly for the LP/HL ewes during lactation. We conclude that an increased intake of MP could enhance the expression of immunity to T. circumcincta of twin-bearing and -rearing Greyface ewes.
在围产期对胃肠道寄生虫的免疫放松（PPRI）具有营养基础这一总体假设下，我们研究了可代谢蛋白质（MP）摄入量的增加是否会影响双胎妊娠和哺乳的灰脸母羊对环形泰勒虫（奥斯特他线虫）的免疫表达。配制了两组等能量日粮，在妊娠最后6周（-P）和泌乳前6周（-L）分别提供MP需求量的85%（低蛋白组，L-）或130%（高蛋白组，H-）。30只母羊被分配到低蛋白组或高蛋白组。每组6只母羊在产羔后屠宰（S(0)）；每组剩余的24只母羊被分配到低蛋白泌乳组（LL）或高蛋白泌乳组（HL），从而在泌乳期形成四种处理组合（LP/LL、LP/HL、HP/LL和HP/HL；n = 12）。每种处理组合的6只母羊在泌乳第3周（S(3)）或第6周（S(6)）屠宰。与低蛋白组母羊相比，高蛋白组母羊产羔时体重更重，所产羔羊体重更重，粪便虫卵计数（FEC）更低，血浆胃蛋白酶原浓度更低，妊娠后期血浆白蛋白浓度更高，皱胃球样白细胞（GL）更多，但在S(0)时虫负荷相似。LP/HL和HP/HL组母羊的计算产奶量高于LP/LL和HP/LL组母羊。在泌乳的前3周，每种处理组合的FEC均增加。与S(?)相比，这种增加与S(3)时皱胃GL减少同时出现。在S(6)时，LP/HL和HP/HL组母羊的未成熟虫比例更高，皱胃GL更多，高于LP/LL和HP/LL组母羊。仅LP/LL组母羊在泌乳的第二个3周期间FEC持续增加，这与S(6)时血浆胃蛋白酶原浓度升高和皱胃GL缺失同时出现。泌乳期间，LP/HL组母羊的血浆胃蛋白酶原浓度迅速下降。我们得出结论，MP摄入量的增加可以增强双胎妊娠和哺乳的灰脸母羊对环形泰勒虫的免疫表达。
