Folch E, Salas A, Prats N, Panés J, Piqué J M, Gelpí E, Roselló-Catafau J, Closa D
Department of Medical Bioanalysis, IIBB-CSIC,IDIBAPS, Barcelona, Spain.
Free Radic Biol Med. 2000 Apr 15;28(8):1286-94. doi: 10.1016/s0891-5849(00)00245-8.
P-selectin and circulating xanthine oxidase are involved in the process of neutrophil infiltration into the lung associated with acute pancreatitis. This study investigated the mediators that trigger the upregulation of P-selectin in this process. Pancreatitis was induced in rats by intraductal administration of 5% sodium taurocholate. P-selectin expression was measured using radiolabeled antibodies. Neutrophil infiltration and PAF levels were also evaluated. The role of superoxide radical, H(2)O(2), or the enzyme poly (ADP-ribose) synthetase (PARS) on these processes was determined in groups of animals treated with the corresponding inhibitors. Pancreatitis was associated with an increase in P-selectin expression in the lung. Inhibition of PARS or H(2)O(2) abrogated P-selectin upregulation, PAF generation, and neutrophil recruitment. Superoxide dismutation prevented neutrophil recruitment and PAF generation, but had no effect on P-selectin expression. We conclude that during acute pancreatitis, upregulation of P-selectin in the pulmonary endothelium is triggered by H(2)O(2) and PARS activity.
P-选择素和循环中的黄嘌呤氧化酶参与了急性胰腺炎相关的中性粒细胞浸润至肺的过程。本研究调查了在此过程中触发P-选择素上调的介质。通过导管内注射5%牛磺胆酸钠在大鼠中诱导胰腺炎。使用放射性标记抗体测量P-选择素的表达。还评估了中性粒细胞浸润和血小板活化因子（PAF）水平。在使用相应抑制剂处理的动物组中确定了超氧阴离子、H₂O₂或酶聚（ADP-核糖）合成酶（PARS）在这些过程中的作用。胰腺炎与肺中P-选择素表达增加有关。抑制PARS或H₂O₂可消除P-选择素上调、PAF生成和中性粒细胞募集。超氧化物歧化可防止中性粒细胞募集和PAF生成，但对P-选择素表达无影响。我们得出结论，在急性胰腺炎期间，肺内皮细胞中P-选择素的上调是由H₂O₂和PARS活性触发的。
