Tuteja R, Li T C, Takeda N, Jameel S
International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.
Viral Immunol. 2000;13(2):169-78. doi: 10.1089/vim.2000.13.169.
DNA vaccines encoding a viral structural protein have been shown to induce antiviral immune responses and provide protection against subsequent viral challenge. In the present study we show that DNA immunization with a plasmid expressing the hepatitis E virus ORF2 structural protein (pcDNA-ORF2) induced low levels of long-lasting antibody responses in the murine model. The use of plasmids expressing interleukin-2 (IL-2) and granulocyte-macrophage colony-stimulating-factor (GM-CSF) in conjunction with pcDNA-ORF2 enhanced the antibody responses generated by pORF-2. We further show that the immune responses generated by plasmid pcDNA-ORF2 can be boosted with virus-like particles composed of the ORF2 protein expressed through a baculovirus expression system.
编码病毒结构蛋白的DNA疫苗已被证明可诱导抗病毒免疫反应，并提供针对后续病毒攻击的保护。在本研究中，我们表明用表达戊型肝炎病毒ORF2结构蛋白的质粒（pcDNA-ORF2）进行DNA免疫在小鼠模型中诱导了低水平的持久抗体反应。将表达白细胞介素-2（IL-2）和粒细胞巨噬细胞集落刺激因子（GM-CSF）的质粒与pcDNA-ORF2联合使用可增强pORF-2产生的抗体反应。我们进一步表明，由通过杆状病毒表达系统表达的ORF2蛋白组成的病毒样颗粒可以增强质粒pcDNA-ORF2产生的免疫反应。
