盘基网柄菌α-D-半乳糖苷酶的性质与发育调控
Properties and developmental regulation of an alpha-d-galactosidase from Dictyostelium discoideum.
作者信息
Kilpatrick D C, Stirling J L
出版信息
Biochem J. 1976 Aug 15;158(2):409-17. doi: 10.1042/bj1580409.
Abstract
An alpha-D-galactosidase was detected in cells of the cellular slime mould, Dictyostelium discoideum, at all stages of development. Its specific activity was highest during early development (interphase), and this accumulation of enzyme appears to require protein synthesis de novo. Its subcellular distribution differs from that of other D. discoideum glycosidases, since most activity was recovered in the soluble fraction. No evidence was obtained for more than one isoenzymic form after subjection of extracts to electrophoresis and various chromatographic procedures. It is excreted from the cell during development, but no evidence was found for an extracellular function for the enzyme.
摘要
在细胞黏菌盘基网柄菌发育的各个阶段的细胞中均检测到一种α-D-半乳糖苷酶。其比活性在发育早期（间期）最高，这种酶的积累似乎需要从头合成蛋白质。其亚细胞分布与盘基网柄菌的其他糖苷酶不同，因为大部分活性存在于可溶部分。提取物经过电泳和各种色谱方法处理后，未获得存在多种同工酶形式的证据。在发育过程中它从细胞中分泌出来，但未发现该酶具有细胞外功能的证据。
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