Shi F, Mochida K, Ogura A, Matsuda J, Suzuki O, Watanabe G, Hutz R J, Tsonis C G, Suzuki A K, Taya K
Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Japan.
Life Sci. 2000;66(25):2489-97. doi: 10.1016/s0024-3205(00)80008-9.
Experiments were conducted to elucidate the mechanisms of active immunization against inhibin on ovarian follicular development and selection in guinea pigs. Estrous cycle was synchronized in experimental guinea pigs by implanting progesterone containing tubes. Antibodies that bound 125I-labeled bovine inhibin were produced by all guinea pigs receiving the inhibin vaccine (recombinant ovine alpha-subunit in oil emulsion) without any effects on duration of the estrous cycle. Active immunization against inhibin increased the plasma concentrations of progesterone during the luteal phase and the plasma concentrations of estradiol but failed to increase the plasma concentration of follicle-stimulating hormone (FSH) during preovulatory period. The treatment also increased the number of corpora lutea (from 1.3+/-0.3 to 7.0+/-1.6 per each ovary), and preovulatory sized follicles (from 1.8+/-0.6 to 7.0+/-1.6 per each ovary), and follicles stained positively for inhibin alpha-subunit (from 2.3+/-0.5 to 6.3+/-1.3 per each ovary) significantly. The results indicate that active immunization against inhibin enhances ovulation rate by affecting the follicle selection and only dominant follicle can be stained for inhibin alpha-subunit in guinea pigs. This study is firstly to provide direct evidence that inhibins play important role in follicle selections in guinea pigs.
进行实验以阐明在豚鼠中主动免疫抑制素对卵巢卵泡发育和选择的机制。通过植入含孕酮的管子使实验豚鼠的发情周期同步。所有接受抑制素疫苗（油乳剂中的重组绵羊α亚基）的豚鼠均产生了与125I标记的牛抑制素结合的抗体，且对发情周期的持续时间没有任何影响。主动免疫抑制素可增加黄体期孕酮的血浆浓度和雌二醇的血浆浓度，但在排卵前期未能增加促卵泡激素（FSH）的血浆浓度。该处理还显著增加了黄体的数量（每个卵巢从1.3±0.3个增加到7.0±1.6个）、排卵前大小的卵泡数量（每个卵巢从1.8±0.6个增加到7.0±1.6个）以及抑制素α亚基染色阳性的卵泡数量（每个卵巢从2.3±0.5个增加到6.3±1.3个）。结果表明，在豚鼠中主动免疫抑制素通过影响卵泡选择来提高排卵率，并且只有优势卵泡才能被抑制素α亚基染色。本研究首次提供了直接证据，证明抑制素在豚鼠卵泡选择中起重要作用。
