Shi F, Ozawa M, Komura H, Watanabe G, Tsonis C G, Suzuki A K, Taya K
Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Japan.
J Reprod Fertil. 2000 Jan;118(1):1-7. doi: 10.1530/jrf.0.1180001.
Experiments were conducted to determine whether neutralizing endogenous inhibin affects follicular development and ovulation rate in guinea-pigs. Eighteen female guinea-pigs bearing 4 week progesterone implants were divided into three groups. At 1 week after removal of the progesterone implants, the animals were given a s.c. injection of 1 ml placebo (saline in oil emulsion; control), or 25 or 50 micrograms inhibin vaccine three times at 4 week intervals. Blood samples were collected once a week throughout the experiment for measuring inhibin antibody titres. After the third injection of inhibin vaccine, blood samples and ovaries were collected on the morning of day 8 after the day of oestrus. Inhibin vaccine increased the ovulation rate in a dose-dependent manner (placebo: 4.2 +/- 0.4; 25 micrograms inhibin vaccine: 6.2 +/- 0.9; 50 micrograms inhibin vaccine: 9.8 +/- 0.9) without any effects on the duration of the oestrous cycle. The results also showed that active immunization against inhibin increased the number of atretic follicles of 300-399 microns in diameter on day 8 after ovulation. The present study is the first to show that the active immunization against inhibin may be a useful method for inducing multiple ovulation in guinea-pigs.
进行实验以确定中和内源性抑制素是否会影响豚鼠的卵泡发育和排卵率。将18只带有4周孕酮植入物的雌性豚鼠分为三组。在取出孕酮植入物1周后，给动物皮下注射1毫升安慰剂（油包盐水乳剂；对照组），或每隔4周注射25或50微克抑制素疫苗三次。在整个实验过程中每周采集一次血样以测量抑制素抗体滴度。在第三次注射抑制素疫苗后，在发情日之后的第8天上午采集血样和卵巢。抑制素疫苗以剂量依赖的方式提高了排卵率（安慰剂组：4.2±0.4；25微克抑制素疫苗组：6.2±0.9；50微克抑制素疫苗组：9.8±0.9），对发情周期的持续时间没有任何影响。结果还表明，对抑制素进行主动免疫会增加排卵后第8天直径为300 - 399微米的闭锁卵泡数量。本研究首次表明，对抑制素进行主动免疫可能是诱导豚鼠多排卵的一种有用方法。
