Jarvinen L Z, HogenEsch H, Suckow M A, Bowersock T L
Department of Veterinary Pathobiology, Purdue University, West Lafayette, IN, USA.
Comp Med. 2000 Jun;50(3):263-9.
We evaluated the efficacy of intranasal administration of Pasteurella multocida toxin (PMT) and a potassium thiocyanate extract of P. multocida (CN) encapsulated in alginate microspheres, compared with unencapsulated PMT and CN antigens, in protection of rabbits against pasteurellosis.
New Zealand male rabbits (n=24) were allotted randomly into four intranasally administered vaccine groups: 1, PMT/CN; 2, microencapsulated PMT/CN with or; 3, without subcutaneous priming; and 4, empty microspheres (control). Blood samples and nasal wash specimens were collected before vaccination and one week after each vaccination (days 7, 21, 35, and 49). Rabbits were primed subcutaneously with either unencapsulated PMT/CN or aluminum hydroxide (control) (day 0), vaccinated intranasally (days 14, 28, and 42), challenged intranasally with live P. multocida (day 56), and necropsied (day 60).
Compared with controls, PMT/CN-immunized rabbits had significantly higher concentrations of serum IgG and IgM, nasal IgG, and bronchoalveolar lavage fluid IgA and IgG against CN. Immunized rabbits had 100% survival rate and low numbers of bacteria in liver and lungs; the control group had 50% survival rate and higher numbers of bacteria (> 4x) per gram of tissue in liver and lungs.
The PMT/CN microspheres stimulated systemic and mucosal immune responses similar in effectiveness (protection) to those in response to unencapsulated PMT/CN administration.
我们评估了藻酸盐微球包裹的多杀巴斯德菌毒素（PMT）和多杀巴斯德菌硫氰酸钾提取物（CN）经鼻给药，与未包裹的PMT和CN抗原相比，在保护兔抵抗巴氏杆菌病方面的效果。
将24只新西兰雄性兔随机分为四个经鼻给药疫苗组：1. PMT/CN；2. 有或无皮下预免疫的微囊化PMT/CN；3. 无皮下预免疫；4. 空微球（对照组）。在接种疫苗前以及每次接种疫苗后一周（第7、21、35和49天）采集血样和鼻腔冲洗标本。兔在第0天皮下注射未包裹的PMT/CN或氢氧化铝（对照）进行预免疫，在第14、28和42天经鼻接种疫苗，在第56天经鼻用活的多杀巴斯德菌攻击，并在第60天进行尸检。
与对照组相比，PMT/CN免疫的兔血清IgG和IgM、鼻腔IgG以及支气管肺泡灌洗液中针对CN的IgA和IgG浓度显著更高。免疫的兔存活率为100%，肝脏和肺部细菌数量少；对照组存活率为50%，肝脏和肺部每克组织中的细菌数量更高（>4倍）。
PMT/CN微球刺激的全身和黏膜免疫反应在有效性（保护作用）上与未包裹的PMT/CN给药所引发的免疫反应相似。
