Suckow Mark A, Jarvinen Lamis Z, HogenEsch Harm, Park Kinam, Bowersock Terry L
400 Freimann Life Science Center, University of Notre Dame, Notre Dame, IN 46556, USA.
J Control Release. 2002 Dec 13;85(1-3):227-35. doi: 10.1016/s0168-3659(02)00277-8.
Pasteurella multocida is an important bacterial pathogen of domestic rabbits. To evaluate the ability of a thiocyanate extract (PTE) of P. multocida to stimulate an immune response and protect against infection with P. multocida, rabbits were immunized subcutaneously or intranasally on Days 7, 21 and 35. Cholera toxin, a potent mucosal adjuvant, was included in one treatment group. Rabbits immunized subcutaneously (SC) or intranasally (IN) had significant increases in serum anti-PTE IgG but not IgA. In contrast, only rabbits immunized IN with PTE developed significant titers of nasal lavage anti-PTE IgA and cholera toxin significantly enhanced this response. In a second study rabbits were immunized via the drinking water with PTE incorporated into alginate microparticles on Days 7, 14 and 21. Mild increases in serum IgG were noted in rabbits immunized with PTE in microparticles, with or without cholera toxin, and this increase was significant (P<or=0.05) on Day 21 for rabbits receiving PTE and cholera toxin. Nasal lavage anti-PTE IgA was significantly (P<or=0.05) increased in rabbits immunized orally with PTE, with or without cholera toxin, in microparticles. This effect was not enhanced by cholera toxin. Rabbits orally immunized with PTE in microparticles had significantly fewer colony forming units of homologous P. multocida recovered from the lungs and nasopharynx following an intranasal challenge. These results demonstrate that PTE incorporated into alginate microparticles and administered orally is immunogenic and confers protective immunity.
多杀性巴氏杆菌是家兔的一种重要细菌病原体。为了评估多杀性巴氏杆菌的硫氰酸盐提取物（PTE）刺激免疫反应及预防多杀性巴氏杆菌感染的能力，在第7、21和35天对家兔进行皮下或鼻内免疫。一个治疗组中包含强效黏膜佐剂霍乱毒素。皮下（SC）或鼻内（IN）免疫的家兔血清抗PTE IgG显著增加，但IgA未增加。相比之下，只有用PTE鼻内免疫的家兔产生了显著滴度的鼻腔灌洗抗PTE IgA，且霍乱毒素显著增强了这种反应。在第二项研究中，于第7、14和21天通过饮用水给家兔免疫掺入藻酸盐微粒中的PTE。在用含或不含霍乱毒素的微粒PTE免疫的家兔中，血清IgG有轻度增加，对于接受PTE和霍乱毒素的家兔，在第21天这种增加具有显著性（P≤0.05）。在用含或不含霍乱毒素的微粒PTE经口免疫的家兔中，鼻腔灌洗抗PTE IgA显著（P≤0.05）增加。霍乱毒素未增强这种效应。经口用微粒PTE免疫的家兔在鼻内攻毒后，从肺和鼻咽中回收的同源多杀性巴氏杆菌的菌落形成单位显著减少。这些结果表明，掺入藻酸盐微粒并经口给药的PTE具有免疫原性并能提供保护性免疫。
