Koranda M, Schleiffer A, Endler L, Ammerer G
Department of Biochemistry and Molecular Cell Biology, Ludwig Boltzmann Forschungsstelle, University of Vienna, Austria.
Nature. 2000 Jul 6;406(6791):94-8. doi: 10.1038/35017589.
Many cell-cycle-specific events are supported by stage-specific gene expression. In budding yeast, at least three different nuclear factors seem to cooperate in the periodic activation of G2/M-specific genes. Here we show, by using chromatin immunoprecipitation polymerase chain reaction assays, that a positive regulator, Ndd1, becomes associated with G2/M promoter regions in manner that depends on the stage in cell cycle. Its recruitment depends on a permanent protein-DNA complex consisting of the MADS box protein, Mcm1, and a recently identified partner Fkh2, a forkhead/winged helix related transcription factor. The lethality of Ndd1 depletion is suppressed by fkh2 null mutations, which indicates that Fkh2 may also have a negative regulatory role in the transcription of G2/M-induced RNAs. We conclude that Ndd1-Fkh2 interactions may be the transcriptionally important process targeted by Cdk activity.
许多细胞周期特异性事件都由阶段特异性基因表达所支持。在芽殖酵母中，至少三种不同的核因子似乎在G2/M特异性基因的周期性激活中协同作用。在此我们通过染色质免疫沉淀聚合酶链反应分析表明，一种正向调节因子Ndd1以依赖于细胞周期阶段的方式与G2/M启动子区域相关联。它的募集依赖于一种由MADS盒蛋白Mcm1和最近鉴定出的伙伴Fkh2（一种叉头/翼状螺旋相关转录因子）组成的永久性蛋白质-DNA复合物。fkh2缺失突变可抑制Ndd1缺失的致死性，这表明Fkh2在G2/M诱导的RNA转录中可能也具有负调节作用。我们得出结论，Ndd1-Fkh2相互作用可能是Cdk活性靶向的转录重要过程。
