du Raan H, Lötter M G, Harris G W, van Aswegen A, Otto A C, Nel M G, Badenhorst P N, Verbeke K A, Verbruggen A M
Department of Medical Physics, University of the Orange Free State, Bloemfontein, South Africa.
Nucl Med Commun. 2000 Jun;21(6):539-44. doi: 10.1097/00006231-200006000-00007.
Red cell volume (RCV) and plasma volume (PV) measurements are performed routinely in nuclear medicine departments to diagnose a number of haematological disorders. Currently, 125I-HSA is used as a plasma tracer and 99Tcm-labelled red cells to determine red cell volume. 125I-HSA is not always readily available, leading to inconvenience for patients and medical practitioners. Due to the availability of 99Tcm in nuclear medicine departments, the use of albumin labelled with 99Tcm was investigated. A new 99Tcm-human serum albumin labelling kit (99Tcm-DMP-HSA) was developed by Verbeke and supplied for use in this study. The main aim of the study was to investigate the use of 99Tcm-DMP-HSA for PV determination. Secondly, the feasibility to determine red cell and plasma volume simultaneously using 99Tcm as radionuclide in both instances was investigated. Fourteen healthy volunteers were enrolled in the dual-phase study. During the first study, 99Tcm-DMP-HSA was used as tracer to calculate PV (PV1a) after intravenous administration. Subsequently, 99Tcm-labelled red cells were administered and the PV (PV1b) and RCV (RCV1) were calculated. The second study was repeated within 2 weeks using the conventional method. 125I-HSA and 99Tcm-labelled red cells were administered simultaneously. The PV (PV2) and RCV (RCV2) were calculated. We found that the redistribution of 99Tcm-DMP-HSA is faster than that of 125I-HSA; therefore, the plasma counts obtained at different times were back-extrapolated to time zero for plasma volume calculations. The mean values for the different calculated PVs were 2964+/-470 ml for PV1a, 3006+/-623 ml for PV1b and 3001+/-530 ml for PV2, the reference PV. The confidence intervals indicate no significant differences between plasma volumes PV1a and PV2 and plasma volumes PV1a and PV1b. The mean calculated RCV1 was 2130+/-322 ml; that of RCV2 was 2128+/-353 ml. The difference between RCV1 and RCV2 was not significant. Our results indicate that 99Tcm-DMP-HSA could be used for plasma volume calculation. Red cell and plasma volumes can be calculated simultaneously using 99Tcm as radionuclide in both cases.
红细胞容积（RCV）和血浆容积（PV）测量在核医学科常用于诊断多种血液系统疾病。目前，125I - 人血清白蛋白（HSA）用作血浆示踪剂，99锝（99Tcm）标记的红细胞用于测定红细胞容积。125I - HSA并非总能轻易获取，给患者和医护人员带来不便。鉴于核医学科有99Tcm，故对99Tcm标记的白蛋白的应用展开研究。Verbeke研发了一种新型99Tcm - 人血清白蛋白标记试剂盒（99Tcm - DMP - HSA）并提供用于本研究。该研究的主要目的是探究99Tcm - DMP - HSA用于测定PV的情况。其次，研究了在两种情况下同时使用99Tcm作为放射性核素测定红细胞和血浆容积的可行性。14名健康志愿者参与了这项双阶段研究。在第一项研究中，静脉注射99Tcm - DMP - HSA作为示踪剂以计算PV（PV1a）。随后，注射99Tcm标记的红细胞并计算PV（PV1b）和RCV（RCV1）。第二项研究在2周内采用传统方法重复进行。同时注射125I - HSA和99Tcm标记的红细胞。计算PV（PV2）和RCV（RCV2）。我们发现99Tcm - DMP - HSA的再分布比125I - HSA更快；因此，将不同时间获得的血浆计数回推至时间零点以进行血浆容积计算。不同计算得到的PV的平均值分别为：PV1a为2964±470 ml，PV1b为3006±623 ml，参考PV（PV2）为3001±530 ml。置信区间表明血浆容积PV1a与PV2之间以及血浆容积PV1a与PV1b之间无显著差异。计算得到的平均RCV1为2130±322 ml；RCV2为2128±353 ml。RCV1与RCV2之间的差异不显著。我们的结果表明99Tcm - DMP - HSA可用于血浆容积计算。在两种情况下均可使用99Tcm作为放射性核素同时计算红细胞和血浆容积。
