McLaughlin B J, Wood J G, Saito K, Roberts E, Wu J Y
Brain Res. 1975 Mar 7;85(3):355-71. doi: 10.1016/0006-8993(75)90813-6.
The immunocytochemical localization of L-glutamate decarboxylase (GAD), the enzyme which which forms gamma-aminobutyric acid (GABA), has been studied in developing rodent cerebellum. During the first 3-4 postnatal days, GAD is distributed along non-terminal portions of axonal processes in close association with small vesicles. Some of the axonal processes emanate from profiles which resemble growth cone varicosities, and are presumed to be foliopodia which extend distally from axonal growth regions. At the end of the first postnatal week the GAD-containing axonal processes are seen to form protosynaptic contacts, and GAD is localized around synaptic vesicles and at presynaptic junctional membranes. During the second and third postnatal weeks GAD gradually becomes localized to mature synaptic terminals in association with synaptic vesicle, mitochondrial, and presynaptic junctional membranes. The results suggest that GAD is present in growing neurites in close association with small vesicles prior to the time the neurites make protosynaptic contacts, and that differentiation of these contacts results in a sequestering of GAD into synaptic terminals.
已对发育中的啮齿动物小脑进行了研究，该酶形成γ-氨基丁酸（GABA），即L-谷氨酸脱羧酶（GAD）的免疫细胞化学定位。在出生后的前3-4天，GAD沿轴突过程的非终末部分分布，与小泡紧密相关。一些轴突过程来自类似生长锥膨体的结构，推测是从轴突生长区域向远端延伸的丝状伪足。在出生后第一周结束时，可见含GAD的轴突过程形成原突触接触，且GAD定位于突触小泡周围和突触前连接膜处。在出生后的第二和第三周，GAD逐渐定位于与突触小泡、线粒体和突触前连接膜相关的成熟突触终末。结果表明，在神经突形成原突触接触之前，GAD存在于与小泡紧密相关的生长神经突中，并且这些接触的分化导致GAD被隔离到突触终末。
