Chan-Palay V, Nilaver G, Palay S L, Beinfeld M C, Zimmerman E A, Wu J Y, O'Donohue T L
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7787-91. doi: 10.1073/pnas.78.12.7787.
Purkinje neurons of the cerebellar cortex from a chemically and morphologically heterogeneous population containing some members that have gamma-aminobutyric acid (GABA), others that have immunoreactivity for motilin, and a small number that have both. The remaining 30-40% of all Purkinje cells have neither of these two neuroactive substances, leaving possibilities for other transmitter candidates. The evidence was compiled from double-staining immunocytochemical procedures performed on single sections of the cerebellum and brain stem in rat, mouse, and monkey. Two polyclonal antibodies were applied in succession, one directed against the midregion and COOH terminus of the 22-amino acid polypeptide motilin and the other against glutamic acid decarboxylase (glutamate decarboxylase; L-glutamate 1-carboxy-lyase, EC 4.1.1.15), the rate-limiting enzyme in the synthesis of the neurotransmitter GABA. The staining combinations employed the immunoperoxidase method, with different chromogens for distinguishing the motilin-like immunoreactivity from glutamic acid decarboxylase immunoreactivity by different colors, or the immunoperoxidase method for one antiserum and immunofluorescence for the other. The locations of both motilin and GABA cell types were mapped. The recognition of motilin in Purkinje cells calls for experimental definition of the role of this substance in the cerebellum and for reevaluation of the roles of Purkinje cells and of GABA in cerebellar function. The significant motilin representation in the flocculus, paraflocculus, and vermis suggests that it may be the Purkinje cell mediative chemical in the vestibular parts of the cerebellum. However, the presence of GABA as well in the same regions indicates that the chemical preference may be at least bimodal.
小脑皮质的浦肯野神经元来自化学和形态上异质的群体，其中一些成员含有γ-氨基丁酸（GABA），另一些对胃动素具有免疫反应性，还有少数同时具有这两种物质。其余30%至40%的浦肯野细胞既不含有这两种神经活性物质，这为其他递质候选物留下了可能性。证据来自对大鼠、小鼠和猴子的小脑和脑干单切片进行的双重染色免疫细胞化学程序。相继应用了两种多克隆抗体，一种针对22个氨基酸多肽胃动素的中部区域和COOH末端，另一种针对谷氨酸脱羧酶（谷氨酸脱羧酶；L-谷氨酸1-羧基裂解酶，EC 4.1.1.15），它是神经递质GABA合成中的限速酶。染色组合采用免疫过氧化物酶法，用不同的发色剂通过不同颜色区分胃动素样免疫反应性和谷氨酸脱羧酶免疫反应性，或者一种抗血清采用免疫过氧化物酶法，另一种采用免疫荧光法。绘制了胃动素和GABA细胞类型的位置图。在浦肯野细胞中识别出胃动素需要对该物质在小脑中的作用进行实验性定义，并重新评估浦肯野细胞和GABA在小脑功能中的作用。在绒球、旁绒球和蚓部中显著的胃动素表现表明，它可能是小脑中前庭部分的浦肯野细胞介导化学物质。然而，同一区域中也存在GABA，这表明化学偏好可能至少是双峰的。
