Enweani C C, Gyles C L, Barnum D A
Can J Comp Med. 1975 Jan;39(1):46-53.
Nineteen antisera produced in pigs against 14 enteropathogenic and five nonenterotoxigenic porcine strains of Escherichia coli were tested for their ability to inhibit gut loop fluid accumulation induced by homologous and heterologous organisms. In addition, four antisera produced in pigs by an intensive series of intravenous inoculations and three by a less intensive series of intramuscular injections of a polyvalent E. coli vaccine were evaluated. Antisera were also produced in rabbits against eight strains of porcine enteropathogens and tested in pig gut loops. Fluid inhibiting activity was detected in prevaccinal sera of pigs but not of rabbits. This activity was significantly increased following immunization. When single strains of E. coli were used for immunization the activity of the antisera against heterologous organisms varied considerably from one test strain to another and was usually much less than that against the homologous organism. The activity against heterologous organisms could not be associated with relatedness of the O, K and H antigens of the vaccine and the test strains. Antisera produced against a vaccine made by combining three strains were shown to exert inhibitory effects on heterologous organisms similar to those against homologous organisms. Considerably less activity against homologous and heterologous organisms was present in antisera produced by the series of intramuscular compared with the series of intravenous injections.
检测了用猪制备的针对14种肠致病性和5种非产毒素性猪源大肠杆菌菌株产生的19种抗血清抑制同源和异源菌株诱导的肠袢积液的能力。此外，还评估了通过一系列密集静脉注射在猪体内产生的4种抗血清，以及通过对多价大肠杆菌疫苗进行较少密集肌肉注射在猪体内产生的3种抗血清。还用兔制备了针对8种猪肠道病原体菌株的抗血清，并在猪肠袢中进行了检测。在猪的疫苗接种前血清中检测到了液体抑制活性，但在兔的血清中未检测到。免疫后这种活性显著增强。当用单一的大肠杆菌菌株进行免疫时，抗血清对异源菌株的活性在不同测试菌株之间差异很大，并且通常远低于对同源菌株的活性。抗血清对异源菌株的活性与疫苗和测试菌株的O、K和H抗原的相关性无关。针对由三种菌株组合制成的疫苗产生的抗血清对异源菌株的抑制作用与对同源菌株的抑制作用相似。与静脉注射系列相比，肌肉注射系列产生的抗血清对同源和异源菌株的活性要低得多。
