García-Vitoria M, García-Corchón C, Rodríguez J A, García-Amigot F, Burrell M A
Departments of Cytology and Histology, University of Navarra, Pamplona, Spain.
J Histochem Cytochem. 2000 Aug;48(8):1111-20. doi: 10.1177/002215540004800808.
The aim of this study was to identify which cell types of the rat gastric epithelium express neuronal nitric oxide synthase (nNOS) because the results of the previous studies have been very divergent regarding this point. By the combination of immunohistochemical (IHC) and in situ hybridization (ISH) techniques, we detected expression of nNOS in chief and mucosecretory cells of the gastric epithelium. Moreover, some gastric endocrine cells were immunoreactive for nNOS, although they could not be distinguished in sections treated with ISH techniques. The strongest signal for all antibodies in IHC techniques was obtained when microwave (MW) heating was performed before the IHC procedure. Our results indicate that in the gastric epithelium a variety of cell types are able to produce NO. The NO produced by the different cell types (chief, mucous, and endocrine) may form a complex network of paracrine communication with an important role in gastric physiology.
本研究的目的是确定大鼠胃上皮的哪些细胞类型表达神经元型一氧化氮合酶（nNOS），因为之前关于这一点的研究结果差异很大。通过免疫组织化学（IHC）和原位杂交（ISH）技术相结合，我们在胃上皮的主细胞和黏液分泌细胞中检测到了nNOS的表达。此外，一些胃内分泌细胞对nNOS有免疫反应，尽管在用ISH技术处理的切片中无法区分它们。在免疫组织化学技术中，当在免疫组织化学程序之前进行微波（MW）加热时，所有抗体获得的信号最强。我们的结果表明，在胃上皮中，多种细胞类型能够产生NO。不同细胞类型（主细胞、黏液细胞和内分泌细胞）产生的NO可能形成一个旁分泌通讯的复杂网络，在胃生理学中起重要作用。
