DNA拓扑结构：感受拓扑异构酶的脉动

DNA topology: feeling the pulse of a topoisomerase.

作者信息

Stasiak A

机构信息

Laboratoire d'Analyse Ultrastructurale, Bätiment de Biologie, Universté de Lausanne, Lausanne-Dorigny, CH-1015, Switzerland.

出版信息

Curr Biol. 2000 Jul 13;10(14):R526-8. doi: 10.1016/s0960-9822(00)00585-6.

DOI:10.1016/s0960-9822(00)00585-6

PMID:10898993

Abstract

The action of individual type II DNA topoisomerases has been followed in real time by observing the elastic response of single DNA molecules to sequential strand passage events. Micromanipulation methods provide a complementary approach to biochemical studies for investigating the mechanism of DNA topoisomerases.

摘要

通过观察单个DNA分子对连续链通过事件的弹性反应，实时跟踪了个体II型DNA拓扑异构酶的作用。显微操作方法为研究DNA拓扑异构酶的机制提供了一种补充生化研究的方法。