Nisolle M, Casanas-Roux F, Qu J, Motta P, Donnez J
Service de Gynécologie, Université Catholique de Louvain, Brussels, Belgium.
Fertil Steril. 2000 Jul;74(1):122-9. doi: 10.1016/s0015-0282(00)00548-3.
To compare histologic and ultrastructural characteristics of fresh and frozen-thawed human ovarian cortical tissue grafted into nude mice.
Experimental prospective study.
An academic research environment.
PATIENT(S): Ovarian biopsy specimens were obtained from 13 women undergoing laparoscopy for tubal ligation or infertility.
ANIMAL(S): Forty nude mice.
INTERVENTION(S): A minilaparotomy was performed to place fresh and frozen-thawed ovarian grafts subcutaneously (sc) or intraperitoneally (ip). Removal of the ovarian grafts was performed at 24 days.
MAIN OUTCOME MEASURE(S): [1] the follicular population, [2] fibrosis, [3] vascularization of the grafted tissue, and [4] ultrastructural evaluation.
RESULT(S): A greater fibrosis relative surface area was noted in frozen-thawed transplanted tissue than in fresh transplants. Regardless of this fibrosis, a similar follicular density was observed in fresh and frozen-thawed ovarian tissue 24 days after transplantation. Active angiogenesis was proved by both immunohistochemical study of the vascular endothelial growth factor and morphometric study of the vascular network. Normal ultrastructural characteristics were noted in frozen-thawed ovarian biopsies.
CONCLUSION(S): Angiogenesis allows implantation of the graft even if it has been cryopreserved and thawed similarly to implantation of fresh tissue. The greater fibrosis observed in grafts after cryopreservation and implantation does not seem to affect the primordial and primary ovocyte population and their ultrastructural characteristics, but further studies must be conducted to prove that after cryopreservation and transplantation, ovocytes may achieve full maturation and fertilization.
比较移植到裸鼠体内的新鲜和冻融人卵巢皮质组织的组织学和超微结构特征。
实验性前瞻性研究。
学术研究环境。
从13名因输卵管结扎或不孕而接受腹腔镜检查的女性获取卵巢活检标本。
40只裸鼠。
通过小型剖腹术将新鲜和冻融的卵巢移植物皮下（sc）或腹腔内（ip）植入。在24天时取出卵巢移植物。
[1]卵泡数量，[2]纤维化，[3]移植组织的血管化，[4]超微结构评估。
与新鲜移植组织相比，冻融移植组织的纤维化相对表面积更大。尽管存在这种纤维化，但在移植后24天，新鲜和冻融卵巢组织中观察到相似的卵泡密度。血管内皮生长因子的免疫组化研究和血管网络的形态计量学研究均证实了活跃的血管生成。冻融卵巢活检标本具有正常的超微结构特征。
血管生成使移植物即使经过冷冻保存和解冻也能像新鲜组织移植一样植入。冷冻保存和植入后移植物中观察到的更大纤维化似乎不影响原始卵母细胞和初级卵母细胞数量及其超微结构特征，但必须进行进一步研究以证明冷冻保存和移植后卵母细胞是否能完全成熟和受精。
