Reproductive Center, First Affiliated Hospital of Shantou University Medical College, Shantou University, Shantou City, 515041, Guangdong, China.
Clin Sci (Lond). 2023 Oct 31;137(20):1577-1591. doi: 10.1042/CS20230483.
The process of ovarian cryopreservation and transplantation is the only feasible fertility preservation method for prepubertal girls and female patients with cancer who cannot delay radiotherapy and chemotherapy. However, basic research on this technique is lacking. To better understand ovarian function and oocyte quality after ovarian tissue (OT) transplantation, we characterised the appearance, angiogenesis, and endocrine function of ovarian grafts in a murine model; the mitochondrial function and DNA damage in oocytes isolated from the OT; and the development of embryos after in vitro fertilisation. The results showed a decrease in oocyte numbers in the transplanted OT, abnormal endocrine function of ovarian grafts, as well as dysfunctional mitochondria and DNA damage in the oocytes, which could adversely affect subsequent embryonic development. However, these adverse phenotypes were partially or completely resolved within 21 days of transplantation, suggesting that ovulation induction and assisted pregnancy treatment should not be conducted too soon after OT transfer to ensure optimal patient and offspring outcomes.
卵巢组织冻存和移植的过程是青春期前女童和因放化疗而无法延迟治疗的癌症女性患者唯一可行的生育力保存方法。然而，该技术的基础研究却相对缺乏。为了更好地了解卵巢组织（OT）移植后的卵巢功能和卵母细胞质量，我们在鼠模型中对卵巢移植物的外观、血管生成和内分泌功能进行了特征描述；对从 OT 中分离出的卵母细胞的线粒体功能和 DNA 损伤进行了研究；并对体外受精后胚胎的发育情况进行了研究。结果显示，移植的 OT 中的卵母细胞数量减少，卵巢移植物的内分泌功能异常，以及卵母细胞中线粒体功能和 DNA 损伤，这些都可能对后续胚胎发育产生不利影响。然而，在移植后 21 天内，这些不良表型部分或完全得到解决，这表明在 OT 移植后不应该过早进行排卵诱导和辅助妊娠治疗，以确保患者和后代的最佳结局。
